Recombinant Human AHR 293 Cell Lysate
Cat.No. : | AHR-8962HCL |
- Specification
- Gene Information
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Description : | Antigen standard for aryl hydrocarbon receptor (AHR) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AHR aryl hydrocarbon receptor [ Homo sapiens ] |
Official Symbol : | AHR |
Synonyms : | AHR; aryl hydrocarbon receptor; bHLHe76; AH-receptor; ah receptor; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76; |
Gene ID : | 196 |
mRNA Refseq : | NM_001621 |
Protein Refseq : | NP_001612 |
MIM : | 600253 |
UniProt ID : | P35869 |
Chromosome Location : | 7p15 |
Pathway : | Adipogenesis, organism-specific biosystem; |
Function : | DNA binding; DNA binding; Hsp90 protein binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; protein binding; protein dimerization activity; protein heterodimerization activity; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; signal transducer activity; transcription factor binding; transcription regulatory region DNA binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThe expression and function of AHR can be influenced by genetic polymorphisms, DNA methylation patterns, and histone modifications, which may have implications in individual susceptibility to environmental toxins.
AHR is expressed in skin cells and plays a role in the response to environmental toxins, UV radiation, and the regulation of skin barrier function.
AHR is essential for normal embryonic development and organogenesis. It participates in the patterning and differentiation of various organs, including the heart and vasculature.
Yes, AHR activation can interfere with hormonal signaling pathways, leading to cross-talk with estrogen receptor signaling, and affecting hormonal homeostasis.
AHR can impact cell cycle progression and apoptosis by modulating the expression of genes involved in these processes, which can have implications in cancer biology.
AHR is involved in the regulation of immune responses and inflammation by affecting the differentiation and function of immune cells, such as T cells and dendritic cells.
AHR activation induces the expression of cytochrome P450 enzymes, particularly CYP1A1 and CYP1B1, which metabolize and detoxify various environmental pollutants and xenobiotics.
AHR forms a complex with co-activators or co-repressors upon ligand binding, leading to the modulation of gene expression. Downstream targets include cytochrome P450 enzymes (CYP1A1, CYP1B1), phase II detoxification enzymes, and cytokines.
AHR activation occurs upon binding to specific ligands, including polycyclic aromatic hydrocarbons (PAHs), halogenated aromatic hydrocarbons (HAHs), and tryptophan derivatives, such as indole-3-carbinol. Endogenous ligands are derived from cellular metabolism and the diet.
AHR activity can be regulated through various post-translational modifications, including phosphorylation and ubiquitination. Protein-protein interactions with chaperones and co-regulators also influence AHR function.
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