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Recombinant Human S100A9, His-tagged

Cat.No.: S100A9-300H
Product Overview: A DNA sequence encoding the human S100A9 (NP_002956.1) (Met 1-Pro 114) was expressed, fused with a polyhistidine tag at the C-terminus.
Description: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity.
Source: Baculovirus-Insect Cells
Species: Human
Tag: His
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Bio-activity: 1. Measured by its ability to bind recombinant human S100A8-his in a functional ELISA.2. Measured by its ability to bind recombinant human his-S100A8 in a functional ELISA.3. Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-30 μg/mL.
Molecular Mass: The recombinant human S100A9 consists of 124 amino acids and predicts a molecular mass of 14.6 kDa. It migrates as an approximately 16 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Purity: > 95 % as determined by SDS-PAGE
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC
Storage: Store it under sterile conditions at -20ºC to -80ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name: S100A9 S100 calcium binding protein A9 [ Homo sapiens (human) ]
Official Symbol: S100A9
Synonyms: S100A9; S100 calcium binding protein A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387; S100A9; S100; MRP-14 calcium-binding protein A9 (calgranulin B); S100 calcium binding protein A9 (calgranulin B); calgranulin B; OTTHUMP00000015331; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain
Gene ID: 6280
mRNA Refseq: NM_002965
Protein Refseq: NP_002956
MIM: 123886
UniProt ID: P06702
Chromosome Location: 1q21
Function: RAGE receptor binding; Toll-like receptor 4 binding; arachidonic acid binding

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