Active Recombinant Rat Cd86 Protein, Fc Chimera

• Cat.No.: Cd86-960R
• Product Overview: Recombinant Rat Cd86 (Accession # O35531), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Rat
• Tag: Fc
• Predicted N Terminal: Val29
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909.The ED50 for this effect is typically 0.075-0.3 µg/mL in the presence of PHA.
• Molecular Mass: Recombinant Rat Cd86, Fc Chimera has a calculated MW of 52 kDa (monomer). In SDS-PAGE migrates as 80-100 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Cd86 CD86 molecule [ Rattus norvegicus ]
• Official Symbol: Cd86
• Synonyms: CD86; CD86 molecule; T-lymphocyte activation antigen CD86; cd86 antigen; membrane glycoprotein; B7-2
• Gene ID: 56822
• mRNA Refseq: NM_020081
• Protein Refseq: NP_064466