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Active Recombinant Human TDGF1, Fc Chimera

Cat.No. : TDGF1-496H
Product Overview : Recombinant Human Teratocarcinoma-Derived Growth Factor 1 encoding the signal peptide and extracellular domain of human Cripto-1 (aa 1-169) was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modifiedhuman 293 cells.
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Cat. No. : TDGF1-496H
Description : Cripto-1 (CR-1), also known as teratocarcinoma-derived growth factor-1 (TDGF-1), is a small glycoprotein that contains a single divergent EGF-like motif as well as a novel cysteine-rich domain termed the Cripto motif. It is the founding member of the EGF-CFC gene family, which is conserved among vertebrates, with homologs in chick, frogs, and zebrafish.
Source : Human 293 cells.
Amino Acid Sequence : LGHQEFARPSRGYLAFRDDSIWPQE EPAIRPRSSQRVPPMGIQHSKELNR TCCLNGGTCM LGSFCACPPSFYGRNCEHDVRKENC GSVPHDTWLPKKCSLCKCWHGQLRC FPQAFLPG CDGLVMDEHLVASRTPELPPSGSSN TKVDKKVEPKSCDKTHTCPPCPAPE LLGGPSVFLFP PKPKDTLMISRTPEVTCVVVDVSHE DPEVKFNWYVDGVEVHNAKTKPREE QYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAP IEKTISKAKGQPREPQVYTLPPSRD ELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKT TPPVLDSDGSFFLYSKLTVDKSRWQ QGNVFSCSVMH EALHNHYTQKSLSLSPGK.
Molecular Mass : Under reducing conditions Cripto-1 – Fc Chimera migrates as a broad band between 45 and 55 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified Cripto-1 - Fc Chimera that has a predicted monomeric molecular mass of 42.8 kDa.
pI : Cripto-1 – Fc Chimera separates into a number of glycoforms with an observed pI between 6.5 and 10 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Cripto-1 - Fc Chimera that has a predicted pI of 8.2.
% Carbohydrate : Purified Cripto-1 – Fc Chimera consists of 5-30% carbohydrate by weight.
Glycosylation : Cripto-1 – Fc Chimera contains N-linked and O-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE and visualized by Coomassie Briliant Blue.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Activity : 200 ng/ml Cripto-1 – Fc Chimera induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells
Gene Name : TDGF1 teratocarcinoma-derived growth factor 1 [ Homo sapiens ]
Synonyms : TDGF1; teratocarcinoma-derived growth factor 1; CR; CRGF; CRIPTO; Cripto-1; Epidermal growth factor-like cripto protein CR1; Cripto-1 growth factor
Gene ID : 6997
mRNA Refseq : NM_003212
Protein Refseq : NP_003203
UniProt ID : P13385
Chromosome Location : 3p21.31
MIM : 187395
Function : growth factor activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Is TDGF1 associated with any genetic disorders? 01/26/2021

Mutations in the TDGF1 gene have been linked to certain congenital disorders. Understanding these genetic associations can provide insights into the underlying mechanisms of these conditions.

How does TDGF1 influence neurodevelopment? 02/11/2020

TDGF1 has been found to play a role in neural development. It is involved in the specification of neural cell fate and the formation of the neural tube during embryogenesis.

Can TDGF1 be a target for drug development? 06/27/2019

Yes, efforts are underway to develop drugs that modulate TDGF1 activity. Targeting TDGF1 could potentially lead to novel therapies for conditions related to cell growth and differentiation.

In what ways does TDGF1 contribute to stem cell biology? 06/04/2019

TDGF1 is involved in the maintenance of pluripotency in embryonic stem cells. Understanding its role could enhance our ability to control and manipulate stem cells for therapeutic purposes.

How can TDGF1 be utilized in cancer diagnostics? 10/09/2016

Researchers are exploring the use of TDGF1 as a biomarker for certain cancers. Detection of elevated TDGF1 levels in patient samples may aid in early cancer diagnosis.

Customer Reviews (3)

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01/13/2020

    the manufacturer can assist in sourcing complementary reagents and tools, such as specific antibodies or detection systems, to complement the use of TDGF1 protein in trials.

    03/26/2018

      manufacturers often have a wealth of knowledge and expertise in the field of TDGF1 protein research.

      08/29/2017

        They may offer educational resources such as technical guides, protocols, and application notes, which can aid researchers in understanding the principles, techniques, and potential applications of TDGF1 protein in their studies.

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