Active Recombinant Human TDGF1, Fc Chimera
Cat.No. : | TDGF1-496H |
Product Overview : | Recombinant Human Teratocarcinoma-Derived Growth Factor 1 encoding the signal peptide and extracellular domain of human Cripto-1 (aa 1-169) was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modifiedhuman 293 cells. |
- Specification
- Gene Information
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Cat. No. : | TDGF1-496H |
Description : | Cripto-1 (CR-1), also known as teratocarcinoma-derived growth factor-1 (TDGF-1), is a small glycoprotein that contains a single divergent EGF-like motif as well as a novel cysteine-rich domain termed the Cripto motif. It is the founding member of the EGF-CFC gene family, which is conserved among vertebrates, with homologs in chick, frogs, and zebrafish. |
Source : | Human 293 cells. |
Amino Acid Sequence : | LGHQEFARPSRGYLAFRDDSIWPQE EPAIRPRSSQRVPPMGIQHSKELNR TCCLNGGTCM LGSFCACPPSFYGRNCEHDVRKENC GSVPHDTWLPKKCSLCKCWHGQLRC FPQAFLPG CDGLVMDEHLVASRTPELPPSGSSN TKVDKKVEPKSCDKTHTCPPCPAPE LLGGPSVFLFP PKPKDTLMISRTPEVTCVVVDVSHE DPEVKFNWYVDGVEVHNAKTKPREE QYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAP IEKTISKAKGQPREPQVYTLPPSRD ELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKT TPPVLDSDGSFFLYSKLTVDKSRWQ QGNVFSCSVMH EALHNHYTQKSLSLSPGK. |
Molecular Mass : | Under reducing conditions Cripto-1 – Fc Chimera migrates as a broad band between 45 and 55 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified Cripto-1 - Fc Chimera that has a predicted monomeric molecular mass of 42.8 kDa. |
pI : | Cripto-1 – Fc Chimera separates into a number of glycoforms with an observed pI between 6.5 and 10 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Cripto-1 - Fc Chimera that has a predicted pI of 8.2. |
% Carbohydrate : | Purified Cripto-1 – Fc Chimera consists of 5-30% carbohydrate by weight. |
Glycosylation : | Cripto-1 – Fc Chimera contains N-linked and O-linked oligosaccharides. |
Purity : | >95%, as determined by SDS-PAGE and visualized by Coomassie Briliant Blue. |
Formulation : | When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
Reconstitution : | It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
Storage : | Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
Activity : | 200 ng/ml Cripto-1 – Fc Chimera induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells |
Gene Name : | TDGF1 teratocarcinoma-derived growth factor 1 [ Homo sapiens ] |
Synonyms : | TDGF1; teratocarcinoma-derived growth factor 1; CR; CRGF; CRIPTO; Cripto-1; Epidermal growth factor-like cripto protein CR1; Cripto-1 growth factor |
Gene ID : | 6997 |
mRNA Refseq : | NM_003212 |
Protein Refseq : | NP_003203 |
UniProt ID : | P13385 |
Chromosome Location : | 3p21.31 |
MIM : | 187395 |
Function : | growth factor activity |
Products Types
◆ Recombinant Protein | ||
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TDGF1-001H | Recombinant Human TDGF1 Protein, MBP-tagged | +Inquiry |
TDGF1-295H | Recombinant Human TDGF1 Protein, His-tagged(C-ter) | +Inquiry |
TDGF1-562H | Recombinant Human TDGF1 Protein, Fc-tagged | +Inquiry |
TDGF1-1048H | Recombinant Human TDGF1 protein(Met1-Thr172), hFc-tagged | +Inquiry |
◆ Lysates | ||
TDGF1-832RCL | Recombinant Rat TDGF1 cell lysate | +Inquiry |
TDGF1-2432HCL | Recombinant Human TDGF1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionMutations in the TDGF1 gene have been linked to certain congenital disorders. Understanding these genetic associations can provide insights into the underlying mechanisms of these conditions.
TDGF1 has been found to play a role in neural development. It is involved in the specification of neural cell fate and the formation of the neural tube during embryogenesis.
Yes, efforts are underway to develop drugs that modulate TDGF1 activity. Targeting TDGF1 could potentially lead to novel therapies for conditions related to cell growth and differentiation.
TDGF1 is involved in the maintenance of pluripotency in embryonic stem cells. Understanding its role could enhance our ability to control and manipulate stem cells for therapeutic purposes.
Researchers are exploring the use of TDGF1 as a biomarker for certain cancers. Detection of elevated TDGF1 levels in patient samples may aid in early cancer diagnosis.
Customer Reviews (3)
Write a reviewthe manufacturer can assist in sourcing complementary reagents and tools, such as specific antibodies or detection systems, to complement the use of TDGF1 protein in trials.
manufacturers often have a wealth of knowledge and expertise in the field of TDGF1 protein research.
They may offer educational resources such as technical guides, protocols, and application notes, which can aid researchers in understanding the principles, techniques, and potential applications of TDGF1 protein in their studies.
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