Recombinant Human FGF23 Protein, His-tagged

• Cat.No.: FGF23-684H
• Product Overview: Recombinant Human FGF23 Protein is produced by Baculovirus expression system. This protein is fused with a His tag.

Specification

• Description: FGF-23, also known as fibroblast growth factor 23, is a member of the FGF family which is responsible for phosphate and vitamin D metabolism. The main function of this protein seems to be regulation of phosphate concentration in plasma. It is secreted by osteocytes in response to elevated calcitriol and acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF-23 interaction with renal proximal tubular epithelium decreases the reabsorption and increases excretion of phosphate. This protein may also suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption. Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The N-terminal sequence analysis is showing that a C-terminal fragment is detected. Recombinant human FGF-23, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 2 mM DTT, 1 mM EDTA, 10 % glycerol.
• Molecular Mass: 26.4 kDa
• Protein length: 25-251 aa
• AA Sequence: ADPYPNASPL LGSSWGGLIH LYTATARNSY HLQIHKNGHV DGAPHQTIYS ALMIRSEDAG FVVITGVMSR RYLCMDFRGN IFGSHYFDPE NCRFQHQTLE NGYDVYHSPQ YHFLVSLGRA KRAFLPGMNP PPYSQFLSRR NEIPLIHFNT PIPRRHTRSA EDDSERDPLN VLKPRARMTP APASCSQELP SAEDNSPMAS DPLGVVRGGR VNTHAGGTGP EGCRPFAKFI HHHHHH
• Endotoxin: < 1.0 EU per 1 ug of protein (determined by LAL method)
• Purity: > 90 % by SDS - PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Absorbance at 280 nm)

Gene Information

• Gene Name: FGF23 fibroblast growth factor 23 [ Homo sapiens ]
• Official Symbol: FGF23
• Synonyms: Fibroblast growth factor 23; FGF23; ADHR; FGFN; HFTC2; HPDR2; HYPF; PHPTC
• Gene ID: 8074
• mRNA Refseq: NM_020638
• Protein Refseq: NP_065689
• UniProt ID: Q9GZV9

Reviews

07/23/2022

Excellent results, high purity.

07/17/2021

Reliable and effective product.

11/26/2020

Quick delivery, product works.

Q&As

What role does FGF23 play in chronic kidney disease?

FGF23 levels are elevated in chronic kidney disease, contributing to mineral imbalance.

How is FGF23 implicated in cardiovascular health?

High FGF23 levels are associated with increased cardiovascular risk.

How does FGF23 regulate phosphate and calcium homeostasis?

It decreases renal phosphate reabsorption and 1,25-dihydroxyvitamin D production.

Are there known genetic mutations that affect FGF23 production or activity?

Mutations in FGF23 or related proteins can lead to dysregulated phosphate metabolism.

What therapeutic strategies are being explored to modulate FGF23 levels?

Strategies include phosphate binders and FGF23 antibodies to manage elevated FGF23 levels.

In which tissues is FGF23 predominantly produced and secreted?

FGF23 is primarily produced in bone.

What is the primary function of FGF23 in the body?

FGF23 regulates phosphate and vitamin D metabolism.