Recombinant Human PRAME Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PRAME Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PRAME-1474H
Product Overview : PRAME MS Standard C13 and N15-labeled recombinant protein (NP_996838) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 57.9 kDa
AA Sequence : MERRRLRGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR ELFPPLFMAA FDGRHSQTLK AMVQAWPFTC LPLGVLMKGQ HLHLETFKAV LDGLDVLLAQ EVRPRRWKLQ VLDLRKNSHQ DFWTVWSGNR ASLYSFPEPE AAQPMTKKRK VDGLSTEAEQ PFIPVEVLVD LFLKEGACDE LFSYLIEKVK RKKNVLRLCC KKLKIFAMPM QDIKMILKMV QLDSIEDLEV TCTWKLPTLA KFSPYLGQMI NLRRLLLSHI HASSYISPEK EEQYIAQFTS QFLSLQCLQA LYVDSLFFLR GRLDQLLRHV MNPLETLSIT NCRLSEGDVM HLSQSPSVSQ LSVLSLSGVM LTDVSPEPLQ ALLERASATL QDLVFDECGI TDDQLLALLP SLSHCSQLTT LSFYGNSISI SALQSLLQHL IGLSNLTHVL YPVPLESYED IHGTLHLERL AYLHARLREL LCELGRPSMV WLSANPCPHC GDRTFYDPEP ILCPCFMPNT RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PRAME preferentially expressed antigen in melanoma [ Homo sapiens (human) ]
Official Symbol : PRAME
Synonyms : PRAME; preferentially expressed antigen in melanoma; MAPE; melanoma antigen preferentially expressed in tumors; cancer/testis antigen 130; CT130; OIP-4; opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen of melanoma; OIP4;
Gene ID : 23532
mRNA Refseq : NM_206955
Protein Refseq : NP_996838
MIM : 606021
UniProt ID : P78395

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