Recombinant Mouse F12 protein, His & T7-tagged

• Cat.No.: F12-774M
• Product Overview: Recombinant Mouse F12 aa. (Phe352~Tyr587 (Accession # Q80YC5)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a glycoprotein coagulation factor that plays an important role in the intrinsic pathway of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that is autoactivated upon contact with negatively charged surfaces or misfolded protein aggregates. Mice lacking the encoded protein have a severe defect in forming stable fibrin clots.
• Source: E. coli
• Species: Mouse
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.1kDa
• Protein length: Phe352~Tyr587
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: F12 coagulation factor XII (Hageman factor) [ Mus musculus (house mouse) ]
• Official Symbol: F12
• Synonyms: HAF; FXII; factor XII; coagulation factor XII; hageman factor
• Gene ID: 58992
• mRNA Refseq: NM_021489.3
• Protein Refseq: NP_067464.2
• UniProt ID: Q80YC5

Reviews

06/23/2023

Trustworthy data source. Valuable for our experiments.

05/22/2022

Accelerates research progress. Exceptional service provider.

03/26/2018

Enables data-driven decisions. A research necessity.

Q&As

What role does F12 play in thrombotic disorders?

Abnormalities in F12 can lead to increased risk of thrombosis and related disorders.

How do genetic variations in the F12 gene affect coagulation and risk of bleeding or clotting disorders?

Genetic mutations in F12 can result in either excessive bleeding or an increased tendency to form clots.

How does F12 interact with other components of the coagulation and fibrinolytic systems?

F12 works in conjunction with other coagulation factors and proteins to regulate clot formation and breakdown.

What is the impact of altered F12 activity on inflammation and immune response?

Altered F12 activity is associated with inflammation and can influence immune responses.

What potential therapeutic applications could arise from targeting F12 in clotting or inflammatory disorders?

Targeting F12 offers potential in treating clotting disorders and reducing inflammation in certain conditions.

How does F12 activation initiate the intrinsic pathway of coagulation?

Upon activation, F12 triggers a series of enzymatic reactions leading to clot formation.

What is the primary function of Factor XII (F12) in the blood coagulation cascade?

Factor XII (F12) activates the intrinsic pathway of the coagulation cascade, crucial for blood clot formation.