Recombinant Mouse Smug1 Protein, Myc/DDK-tagged

• Cat.No.: Smug1-5983M
• Product Overview: Purified recombinant protein of mouse full-length single-strand selective monofunctional uracil DNA glycosylase (Smug1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 30.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Smug1 single-strand selective monofunctional uracil DNA glycosylase [ Mus musculus (house mouse) ]
• Official Symbol: Smug1
• Synonyms: SMUG1; single-strand selective monofunctional uracil DNA glycosylase; C85220; 1200013B09Rik; A930006H09Rik
• Gene ID: 71726
• mRNA Refseq: NM_027885
• Protein Refseq: NP_082161
• UniProt ID: Q6P5C5