Active Recombinant Mouse Insulin-Like Growth Factor Binding Protein 7, His-tagged

• Cat.No.: Igfbp7-5645M
• Product Overview: RecombinantMouse Igfbp7 protein was expressed in Spodopterafrugiperda. Ser28-Leu281,with an N-terminal 10-His tag.

Specification

• Cat. No.: Igfbp7-5645M
• Description: Igfbp7 isa 31-37 kDa secreted glycoprotein that belongs to the IGFBP superfamily ofmolecules. Members of this superfamily are characterized by the presence of10-12 conserved cysteines in the first third of the molecule, and the partialconservation of an xCGCCxxC octapeptide motif that exists in an N-terminalIGFBP domain. Mouse Igfbp7 cDNA encodes a 281 amino acid (aa) precursorprotein that contains a putative 25 aa signal peptide and a 256 aa matureregion.
• Source: Spodoptera frugiperda, Sf 21(baculovirus) derived.
• Form: Lyophilized from a0.2 um filtered solution in PBS.
• N-terminalSequence: His
• MolecularWeight: 27.5 kDa
• Activity: Measuredby its ability to bind 6Ckine/CCL21 in a functional ELISA. When rmIGFBP-rp1/Igfbp7 isImmobilized at 500 ng/mL (10 μL/well), the concentration of rhCCL21/6Ckinethat produces 50% optimal binding response is found to be approximately 4-20ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 35 kDa,reducing conditions.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use a manual defrost freezer and avoid repeated freeze-thawcycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Igfbp7 insulin-like growth factorbinding protein 7 [ Mus musculus ]
• Official Symbol: Igfbp7
• Synonyms: Igfbp7; insulin-likegrowth factor binding protein 7; AGM;Fstl2; Mac25; insulin-like growth factor-binding protein 7; IBP-7; IGFBP-7;angiomodulin; IGF-binding protein 7
• Gene ID: 29817
• mRNA Refseq: NM_001159518
• Protein Refseq: NP_001152990
• UniProt ID: Q61581
• Chromosome Location: 5
• Pathway: Senescence andAutophagy
• Function: growth factorbinding; insulin-like growth factor binding