Active Recombinant Mouse Fibroblast Growth Factor 6

• Cat.No.: Fgf6-527M
• Product Overview: RecombinantMouse Fgf6 protein was expressed in E. coli. Pro39-Ile208.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 39-208 a.a.
• Description: Fgf6 is a 25-28kDa member of the FGF family of heparin binding polypeptides which are potentregulators of cell proliferation, differentiation, and function. FGF proteinscontain a 120 amino acid (aa) core FGF domain that exhibits a β-trefoil structure.Mature mouse Fgf6 is a 171aa protein that shares 94% and 99% aa sequence identity with human and rat Fgf6, respectively.It binds and signals primarily through FGF R1c, 2c, and 4. Fgf6 functionsas a mitogen for fibroblasts, vascular endothelial cells, and prostatecarcinoma cells, and N-linked glycosylation is required for the fullmitogenic effect. Fgf6 expression is restricted to skeletalmuscle during development, although it can be upregulated in prostate cancerand Kaposi sarcoma.
• Form: Lyophilizedfrom a 0.2 um filtered solution in MOPS, Ammonium Sulfate, EDTA, DTT with BSAas a carrier protein.
• N-terminalSequence: Pro39
• MolecularWeight: 18.8 kDa
• Activity: Measuredin a cell proliferation assay using NR6R-3T3 mouse fibroblasts. The ED50 forthis effect is typically 0.2-1.0 ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 19 kDa,reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Fgf6 fibroblast growth factor 6[ Mus musculus ]
• Official Symbol: Fgf6
• Synonyms: Fgf6; fibroblastgrowth factor 6; Fgf-6; HSTF-2; HST-2; HBGF-6; heparin-binding growthfactor 6; heparin secretory-transforming protein 2
• Gene ID: 14177
• mRNA Refseq: NM_010204
• Protein Refseq: NP_034334
• UniProt ID: P21658
• Chromosome Location: 6
• Pathway: Downstream signalingof activated FGFR; ESC Pluripotency Pathways; FGFR ligand binding andactivation; FGFR1 ligand binding and activation; FGFR1c ligand binding andactivation; FGFR2 ligand binding and activation; FGFR2c ligand binding andactivation; FGFR3 ligand binding and activation; FRS2-mediated cascade;IRS-mediated signalling; Insulin receptor signalling cascade; MAPK signalingpathway; Melanoma; PI-3K cascade; Pathways in cancer; Regulation of ActinCytoskeleton
• Function: growth factoractivity