Recombinant Human P21 Protein (Cdc42/Rac)-Activated Kinase 1, GST-tagged

• Cat.No.: PAK1-1070
• Product Overview: Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The PAK1 has been activated in vivo.

Specification

• Cat. No.: PAK1-1070
• Description: PAK1 is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, CDC42 and RAC. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3. PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 95kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1mM PMSF, 25% glycerol.
• Specific Activity: 74nmol/min/mg
• Purity: >95%
• Concentration: 0.1 ug/ul
• Sequences: Full Length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Axon guidance; Chemokine signaling pathway; Epithelial cell signaling in Helicobacter pylori infection; ErbB signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; MAPK signaling pathway; Natural killer cell mediated cytotoxicity; Regulation of actin cytoskeleton; Renal cell carcinoma; T cell receptor signaling pathway; Axon guidance; Signaling in Immune system

Gene Information

• Gene Name: PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ]
• Official Symbol: PAK1
• Synonyms: PAK1; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; PAKalpha; MGC130000; MGC130001; STE20 homolog, yeast; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); Serine/threonine-protein kinase PAK 1; EC 2.7.11.1; PAK-1; p65-PAK; Alpha-PAK
• Gene ID: 5058
• mRNA Refseq: NM_001128620
• Protein Refseq: NP_001122092
• MIM: 602590
• UniProt ID: Q13153
• Chromosome Location: 11q13-q14
• Function: ATP binding; collagen binding; nucleotide binding; protein serine/threonine kinase activity; transferase activity