||Recombinant Human full length Carbonic Anhydrase I (Carbonate dehydratase I, CA-I, Carbonic anhydrase B) was cloned from human cDNA and expressed inE.coli.It consists of the human Carbonic Anhydrase I (Swiss prot accession: P00915). MW = 29.3 kDa.
||Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.
||> 95% by SDS-PAGE. The protein was observed as a single band migrating at molecular weight about 30 kDa.
||>100 pmole/min/ug measured monitoring the esterase activity of the enzyme at 405nm with 1mM 4-nitrophenyl acetate in 12.5mM Tris, 75mM NaCl, pH 7.5.
||2mg/ml in Tris buffer 20mM pH 7.5, NaCl 150mM. The concentration is calculated from the absorbance at 280nm (e280 = 44920 M-1 cm-1 ).
||Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 1mM.
||-20ºC. The protein is stable at 4 ºC for weeks and at 25 ºC for at least several days. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.