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Recombinant Human Fms-related Tyrosine Kinase 4, His-tagged

Cat.No. : FLT4-161H
Product Overview : Recombinant Human FLT4 produced inInsect cellsfused with a carboxy-terminal 6X his-tag. The recombinant mature sVEGFR-3/FLT-4 is a glycosylated monomeric protein. The sVEGFR-3/FLT-4 monomers have a mass of approximately 120kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Glu774).
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Cat. No. : FLT4-161H
Description : The FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stages of development. It is important for lymphangiogenesis. All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis.
Source : Insect cells.
Subunit : Monomeric glycoprotein.
Purity : >90%, by SDS-PAGE and visualised by silver stain.
Endotoxin Level : <0.1ng per μg of sVEGFR-3.
Buffer : PBS.
Formulation : Lyophilized.
Biologically Activity : Measured by its ability to bind recombinant rat VEGF-C in a functional solid phase binding assay. Immobilised recombinant human sVEGFR-3/FLT-4 at 5μg/ml can bind recombinant rat VEGF-C in a linear range of 8-500ng/ml.
Reconstitution : The lyophilised sVEGFR-3/FLT-4 is soluble in water and most aqueous buffers. The lyophilised sVEGFR-3/FLT-4 should be reconstituted in PBS or medium to a concentration not lower than 100μg/ml.
Stability : Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted sVEGFR-3/FLT-4 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Gene Name : FLT4 fms-related tyrosine kinase 4 [ Homo sapiens ]
Synonyms : FLT4; fms-related tyrosine kinase 4; PCL; FLT41; LMPH1A; VEGFR3; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; vascular endothelial growth factor receptor 3; EC 2.7.10.1; 3` partial; Tyrosine-protein kinase receptor FLT4; VEGFR-3
Gene ID : 2324
mRNA Refseq : NM_002020
Protein Refseq : NP_002011
MIM : 136352
UniProt ID : P35916
Chromosome Location : 5q34-q35
Pathway : Cytokine-cytokine receptor interaction; Focal adhesion; Signaling by VEGF
Function : ATP binding; growth factor binding; nucleotide binding; receptor activity; transferase activity; vascular endothelial growth factor receptor activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Does FLT4 protein correlate with tumor-related markers? 09/14/2022

FLT4 protein is associated with various tumor-related markers such as malignant lymphoma, breast cancer, and lung cancer.

Is there a relationship between FLT4 protein and lymphedema? 04/16/2022

FLT4 protein is a key regulator of lymphedema and plays an important role in lymphangiogenesis and lymphatic drainage.

Is FLT4 protein associated with congenital lymphedema? 06/01/2021

Yes, mutations in the FLT4 protein can lead to congenital lymphedema, a condition caused by abnormal lymphatic vessel development.

What is the potential of FLT4 protein in cancer treatment? 10/16/2020

The protein is considered an important target in anticancer therapy, and inhibition of its activity may reduce tumor angiogenesis and metastasis.

What is the role of FLT4 protein in pulmonary hypertension? 07/16/2020

FLT4 protein plays an important role in pulmonary hypertension, and upregulation of its activity may lead to pathological changes such as vascular remodeling and pulmonary artery stenosis.

What is the role of FLT4 protein in angiogenesis? 04/21/2019

This is involved in the regulation of angiogenesis and lymphangiogenesis by binding to vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor D (VEGF-D).

Customer Reviews (3)

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Reviews
09/05/2022

    The catalytic activity of FLT4 is very strong, which can significantly improve the selectivity and yield of chemical reactions, and its application potential is great.

    09/26/2019

      We were reassured that the quality of FLT4 was consistent.

      03/23/2019

        Using FLT4 for experiments, I can focus more on experimental design and data analysis without worrying about the reproducibility of experimental results.

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