Recombinant Human Cathepsin L2, His-tagged

• Cat.No.: CTSL2-681H
• Product Overview: Recombinant human procathepsin V produced ininsect cellsis fused to a His-tag. 37kDa (SDS-PAGE).

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: Recombinant glycosylated procathepsin V cloned from human cDNA and purified as full-length proenzyme. Can be activated in one step. Cathepsin V, a member of the papain family of cysteine proteases, has 78% identity to cathepsin L, but unlike cathepsin L is not widely expressed, being localized to thymus, testis, corneal epithelium, and macrophages. It is a strong elastase, also cleaving proteins such as the invariant chain (Ii), plasminogen, and kininogen. It is implicated in disease states such as cancer, angiogenesis, atherosclerosis, and myasthenia gravis.
• Source/Host: insect cells.
• Application: Study enzyme kinetics, cleave target substrates and screen for inhibitors.
• Concentration: 0.5mg/ml.
• Purity: ≥90%.
• Specific Activity: 436 U/μg protein. One unit will hydrolyze one pmole Z-Leu-Arg-AMC substrate (Prod. No. BML-P229, 25 μM) per minute at 25°C, in 25mM NaOAc pH 5.5, 100mM NaCl, 1.0mM DTT.
• Formulation: Liquid. In 25mM TRIS-HCl, pH 8.0, containing 100mM sodium chloride, 0.05% Tween-20 and 10% glycerol.
• Long Term Storage: -80°C.
• Use/Stability: Avoid extended periods unfrozen. This enzyme is stable for 6 months when stored as received under the above conditions.
• Handling: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: CTSL2 cathepsin L2 [ Homo sapiens ]
• Synonyms: CTSL2; cathepsin L2; CTSU; CTSV; CATL2; MGC125957; EC3.4.22.43; Cathepsin L2; Cathepsin V; Cathepsin U; UNQ268/PRO305
• Gene ID: 1515
• mRNA Refseq: NM_001333
• Protein Refseq: NP_001324
• MIM: 603308
• UniProt ID: O60911
• Chromosome Location: 9q22.2
• Pathway: Lysosome
• Function: cysteine-type endopeptidase activity; peptidase activity; protein binding