| Conjugation/Label : |
Biotin |
| Description : |
Neo-N-glycoproteins are proteins conjugated with a single type of asparagine (Asn)-linked N-glycan via a linker approximately 40 Å in length. Because they feature well-defined, homogenous N-glycans, neo-N-glycoproteins have advantages over other natural and recombinant glycoprotein standards. |
| Form : |
Lyophilized in PBS (137 mM NaCl, 2.7 mM KCl, 2 mM KH₂PO₄, 10.1 mM Na₂HPO₄, pH 7.4) |
| Molecular Mass : |
~85,000 |
| Applications : |
Western/lectin blots, ELISA, flow cytometry, lectin microarrays, etc. This biotinylated version enables detection using streptavidin conjugates. |
| Notes : |
These products are for research use only and not for resale or for any use in the manufacture of a therapeutic or for any diagnostic purpose. |
| Storage : |
At -20 centigrade for up to 12 months. |
| Reconstitution : |
Reconstituted in 50 μL molecular grade water to yield a 1 mg/mL solution. Once reconstituted, store at 4 centigrade for up to 1 month or at -20 centigrade for up to 12 months. |
| Usage : |
1. For ELISA, coat microtiter wells with 1-2 μg/mL of G2S2(α2,3)N-BSA in preferred buffer for 1 h at 37 centigrade. Block wells, then incubate with samples followed by detection reagents.
2. For Western/lectin blotting, load 1 μg of G2S2(α2,3)N-BSA alongside your samples in SDS-PAGE. Transfer to nitrocellulose membrane and probe with detection reagents.
3. For GlycoSense Array Kit control, use 2-10 μg/mL of biotinylated G2S2(α2,3)N-BSA in preferred incubation buffer. Detect binding using fluorescent streptavidin conjugate. |
