Recombinant Human AFP Protein, MYC/DDK-tagged, C13 and N15-labeled
Cat.No. : | AFP-082H |
Product Overview : | AFP MS Standard C13 and N15-labeled recombinant protein (NP_001125) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
- Specification
- Gene Information
- Related Products
Description : | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
Source : | HEK293 |
Species : | Human |
Tag : | Myc/DDK |
Molecular Mass : | 68.7 kDa |
AA Sequence : | MKWVESIFLIFLLNFTESRTLHRNE YGIASILDSYQCTAEISLADLATIF FAQFVQEATYKEVSKMVKDALTAIE KPTGDEQSSGCLENQLPAFLEELCH EKEILEKYGHSDCCSQSEEGRHNCF LAHKKPTPASIPLFQVPEPVTSCEA YEEDRETFMNKFIYEIARRHPFLYA PTILLWAARYDKIIPSCCKAENAVE CFQTKAATVTKELRESSLLNQHACA VMKNFGTRTFQAITVTKLSQKFTKV NFTEIQKLVLDVAHVHEHCCRGDVL DCLQDGEKIMSYICSQQDTLSNKIT ECCKLTTLERGQCIIHAENDEKPEG LSPNLNRFLGDRDFNQFSSGEKNIF LASFVHEYSRRHPQLAVSVILRVAK GYQELLEKCFQTENPLECQDKGEEE LQKYIQESQALAKRSCGLFQKLGEY YLQNAFLVAYTKKAPQLTSSELMAI TRKMAATAATCCQLSEDKLLACGEG AADIIIGHLCIRHEMTPVNPGVGQC CTSSYANRRPCFSSLVVDETYVPPA FSDDKFIFHKDLCQAQGVALQTMKQ EFLINLVKQKPQITEEQLEAVIADF SGLLEKCCQGQEQEVCFAEEGQKLI SKTRAALGVTRTRPLEQKLISEEDL AANDILDYKDDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : | 50 μg/mL as determined by BCA |
Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
Gene Name : | AFP alpha fetoprotein [ Homo sapiens (human) ] |
Official Symbol : | AFP |
Synonyms : | AFP; alpha fetoprotein; AFPD; FETA; HPAFP; alpha-fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin |
Gene ID : | 174 |
mRNA Refseq : | NM_001134 |
Protein Refseq : | NP_001125 |
MIM : | 104150 |
UniProt ID : | P02771 |
Products Types
◆ Recombinant Protein | ||
AFP-1059H | Recombinant Human AFP Protein (Met1-Val609), HlgG1 Fc-tagged | +Inquiry |
AFP-289H | Recombinant Human AFP Protein, His (Fc)-Avi-tagged | +Inquiry |
AFP-545H | Recombinant Human AFP Protein, MYC/DDK-tagged | +Inquiry |
AFP-52H | Recombinant Human AFP Protein, His-tagged | +Inquiry |
Afp-552M | Recombinant Mouse Afp Protein, MYC/DDK-tagged | +Inquiry |
◆ Native Protein | ||
AFP-8027H | Native Human Alpha FetoProtein | +Inquiry |
AFP-1180H | Native Human Alpha-Fetoprotein | +Inquiry |
AFP-3018P | Native pig AFP | +Inquiry |
◆ Lysates | ||
AFP-1706HCL | Recombinant Human AFP cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (10)
Ask a questionThe clinical utility of AFP protein as a diagnostic or prognostic marker can be validated through clinical studies using patient samples, correlation analyses with disease outcomes, or comparison with existing diagnostic methods.
Downstream signaling pathways or cellular responses triggered by AFP protein binding or activation can be studied using techniques such as Western blotting, gene expression analysis, or functional assays specific to the signaling pathways of interest.
Therapeutic interventions or treatment strategies targeting AFP protein can be evaluated for efficacy and safety using in vitro or in vivo models, disease-specific assays, or clinical trials to assess their impact on disease progression, patient outcomes, or potential side effects.
The cellular and molecular mechanisms underlying the production and secretion of AFP protein can be elucidated through techniques such as gene expression analysis, protein trafficking studies, or immunofluorescence microscopy.
Genetic variations or mutations in the AFP gene that impact its expression or function can be investigated through genetic association studies, functional characterization of mutant variants, or population-based analyses.
The regulatory factors or signaling pathways that control the expression and transcriptional regulation of the AFP gene can be studied using techniques such as promoter analysis, transcription factor binding assays, or gene knockout/knockdown approaches.
The functional roles of AFP protein in embryonic development, tissue regeneration, or disease progression can be investigated through genetic or pharmacological perturbations, in vitro or in vivo model systems, or functional assays specific to the biological processes of interest.
Specific biomarkers or imaging techniques for detecting AFP protein in biological samples can be developed and their sensitivity and specificity can be assessed using techniques such as ELISA, immunohistochemistry, or imaging modalities like MRI or PET.
The interactions of AFP protein with its binding partners or ligands can be studied using techniques such as co-immunoprecipitation, surface plasmon resonance, or receptor-binding assays.
The immune-related functions of AFP protein, such as immunomodulation or immune evasion, can be investigated using techniques like immune cell assays, cytokine profiling, or immune response assays.
Customer Reviews (3)
Write a reviewInvestigating protein-protein interactions in cellular stress responses for cell survival.
Dissecting protein-protein interactions in viral replication for antiviral strategies.
Mapping protein-protein interactions in protein degradation pathways for proteostasis.
Ask a Question for All AFP Products
Required fields are marked with *
My Review for All AFP Products
Required fields are marked with *
Inquiry Basket