Recombinant Human BMPR1A, His-tagged

• Cat.No.: BMPR1A-160H
• Product Overview: Recombinant BMPR1A produced ininsect cellswas a 23kDa protein It consisted an extracellular domain that was fused with a carboxy-terminal 6X his-tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A (this protein) and BMPR1B and the type II receptor BMPR2. Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor 1A (BMPR-1A), also known as activin receptor-like kinase (ALK)-3, is a one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-b family cytokines. In contrast to the TGF-b receptor system in which the type I receptor does not bind TGF-b in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IA binds BMP-2 and -4 with high-affinity in solution and is a potent BMP-2/4 antagonist in vitro.
• Purity: >90% by SDS-PAGE and silver staining.
• Endotoxin level: <0.1ng per μg of BMPRIA.
• Buffer: PBS.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biologically Activity: Measured by its ability to inhibit recombinant human BMP-2 induced alkaline phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 1-3 μg/ml in the presence of 500 ng/ml of recombinant human BMP-2.
• Reconstitution: It is recommended that sterile PBS containing at least 0.1% HSA or BSA be added to the vial to prepare a stock solution of no less than 100 μg/ml of BMPR-IA.
• Stability: Lyophilized samples are stable for greater than six month at -20°C to -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at -2°C to -4°C for one month or at -20°C to -70°C for three month without detecable loss of activity. Avoid repeated freeze–thaw cyles.

Gene Information

• Gene Name: BMPR1A bone morphogenetic protein receptor, type IA [ Homo sapiens ]
• Synonyms: BMPR1A; bone morphogenetic protein receptor, type IA; ALK3; SKR5; CD292; ACVRLK3; 10q23del; OTTHUMP00000020014; activin A receptor, type II-like kinase 3; serine/threonine-protein kinase receptor; Bone morphogenetic protein receptor type-1A; EC 2.7.11.30; Serine/threonine-protein kinase receptor R5; Activin receptor-like kinase 3; ALK-3
• Gene ID: 657
• mRNA Refseq: NM_004329
• Protein Refseq: NP_004320
• MIM: 601299
• UniProt ID: P36894
• Chromosome Location: 10q22.3
• Pathway: Cytokine-cytokine receptor interaction; TGF-beta signaling pathway; Signaling by BMP
• Function: ATP binding; SMAD binding; magnesium ion binding; nucleotide binding; protein homodimerization activity; protein serine/threonine kinase activity; receptor activity; transferase activity; transforming growth factor beta receptor activity