Recombinant Human TEK Tyrosine Kinase, Endothelial

• Cat.No.: TEK-1311H
• Product Overview: Recombinant humanTEK monomer has a calculated molecular mass of approximately 105 kDa, but asa result of glycosylation, the monomer migrates as an approximately 125 kDaprotein in SDS-PAGE under reducing conditions.

Specification

• Cat. No.: TEK-1311H
• Description: The molecularweight of the dimer is 250 kDa. The soluble receptor protein consists of thefull extracellular domain (Met1-Val730). TIE-1 (tyrosine kinase with Ig andEGF homology domains 1) and TIE2/TEK comprise a receptor tyrosine kinase(RTK) subfamily with unique structural characteristics: twoimmunoglobulin-like domains flanking three epidermal growth factor (EGF)-likedomains and followed by three fibronectin type III-like repeats in theextracellular region and a split tyrosine kinase domain in the cytoplasmicregion. These receptors are expressed primarily on endothelial andhematopoietic progenitor cells and play critical roles in angiogenesis,vasculogenesis and hematopoiesis. Human TIE2 cDNA encodes a 1124 amino acidresidue precursor protein with an 18 residue putative signal peptide, a 727residue extracellular domain and a 354 residue cytoplasmic domain. Twoligands, angiopoietin 1 and angiopoietin 2, which bind TIE2 withhigh-affinity have been identified.
• Source: Insect cells
• Species: Human
• Molecule Weight: 250 kDa glycosylateddimer
• Form: Lyophilized from PBS
• Purity: > 90% asdetermined by SDS-PAGE and visualized by silver stain
• Endotoxin Level: < 0.1 ng/µg ofsTIE2/Fc
• Biological Activity: Measuredin a functional ELISA assay. When TIE2/Fc is immobilized at 4 μg/ml(100ul/well), it binds recombinant human Angiopoietin 2 with a linear rangeof 2-100 ng/ml.
• OfficialSymbol: TEK
• Pathways: Angiogenesis;Angiopoietin receptor Tie2-mediated signaling; Cell surface interactions atthe vascular wall; Hemostasis; Rheumatoid arthritis; Tie2 Signaling

Gene Information

• Gene Name: TEK TEK tyrosine kinase,endothelial [ Homo sapiens ]
• Synonyms: TEK; TEK tyrosinekinase, endothelial; TIE2; VMCM; TIE-2; VMCM1; CD202B; angiopoietin-1receptor; hTIE2; p140 TEK; OTTHUMP00000021167; OTTHUMP00000227067;OTTHUMP00000227068; OTTHUMP00000227069; soluble TIE2 variant 1; soluble TIE2variant 2; tyrosine-protein kinase receptor TEK; tunica interna endothelialcell kinase; tyrosine-protein kinase receptor TIE-2; EC 2.7.10.1
• Gene ID: 7010
• mRNA Refseq: NM_000459
• Protein Refseq: NP_000450
• MIM: 600221
• UniProt ID: Q02763
• Chromosome Location: 9p21
• Function: ATP binding;nucleotide binding; protein binding; protein kinase activity; proteintyrosine kinase activity; receptor activity; transmembrane receptor proteintyrosine kinase activity