"THBS4" Related Products

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Recombinant Human Thrombospondin 4, His-tagged

Cat. No.: THBS4-507H
Product Overview: Recombinant Human THBS4protein was expressed in CHO with a C-terminal 10-His tag. Ala22-Asn961.
Description: THBS4 is a 140 kDacalcium-binding protein that interacts with other extracellular matrixmolecules and modulates the activity of various cell types. TSP1 and 2constitute subgroup A and form disulfide-linked homotrimers. The human THBS4cDNA encodes a 961 amino acid precursor that includes a 26 aa signal sequencefollowed by an N-terminal heparin-binding domain, a coiled-coil motif, fourEGF-like repeats, seven TSP type3 repeats, and a TSP C-terminal domain.
Source: Chinese HamsterOvary cell line, CHO-derived.
Form: Lyophilized from a0.2 μm filtered solution in Tris, NaCl and CaCl2.
N-terminalSequence: Ala22
Molecular Weight: 105 kDa
Activity: Measuredby the ability of the immobilized protein to support the adhesion of SVEC410mouse endothelial cells. rh THBS4 is coated to 96 well plates at 10 μg/mL(100 μL/well) overnight then reduced with 20 mM DTT for 30 minutes. When 2 x104 cells/ well are added to rh THBS4 coated plates, approximately50-70% will adhere after one hour at 37°C.
Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
SDS-PAGE: 145 kDa,reducing conditions.
Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: 100 μg/ mLin sterile PBS
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.
Gene Name: THBS4 thrombospondin 4 [ Homosapiens ]
Official Symbol: THBS4
Synonyms: THBS4; thrombospondin4; TSP4; thrombospondin-4; OTTHUMP00000135313; OTTHUMP00000222343
Gene ID: 7060
mRNA Refseq: NM_003248
Protein Refseq: NP_003239
MIM: 600715
UniProt ID: P35443
Chromosome Location: 5q13
Pathway: ECM-receptor interaction; Focal Adhesion;Focal adhesion; Malaria; Phagosome; Phagosome; Signal Transduction; Signalingby PDGF; TGF-beta signaling pathway
Function: calcium ion binding; collagen binding;fibronectin binding; heparin binding; integrin binding; laminin-1 binding;structural molecule activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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