| Cat. No. : |
Hc-4909M |
| Description : |
Mouse Hc is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse Hc shares 60% and 82% amino acid sequence identity to human and rat Hc, respectively. Hc binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. |
| Biological Activity : |
ED₅₀ 1-3 nM as determined by enzyme release assay of myeloperoxidase. |
| Molecular Weight : |
9 kDa |
| Source : |
E. coli |
| Species : |
Mouse |
| Form : |
Lyophilized without additives. |
| Appearance : |
Lyophilized protein |
| Endotoxin Level : |
< 0.1 ng/μg |
| Reconstitution : |
Reconstitute to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C. |
| Purity : |
>95% by SDS-PAGE |
| Storage : |
Store at -20°C or -70°C as supplied. Stable for at least 1 year. After reconstition, store at -70°C. |
| Pathways : |
Activation of C3 and C5; Complement Activation; Complement and Coagulation Cascades; Complement cascade; G alpha (i) signalling events; GPCR downstream signaling; GPCR ligand binding; Herpes simplex infection; Immune System; Innate Immunity Signaling; Peptide ligand-binding receptors; Pertussis; Prion diseases; Signaling by GPCR; Staphylococcus aureus infection |
