"TNFRSF1A" Related Products


Recombinant Human TNFRSF1A, Fc Chimera

Cat. No. : TNFRSF1A-152H
Product Overview : Recombinant Humantumor necrosis factor receptor 1 was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modifiedhuman 293 cells.
Description : Tumor necrosis factor alpha (TNF-a ; TNF alpha) and the structurally related lymphotoxin alpha are pro-inflammatory cytokines produced by a variety of immune and non-immune cells in response to multiple stimuli. These stimuli can include bacteria and bacterial products such as LPS, viral infections, T-cell receptor activation and cross-linking of surface immunoglobulin (Ig) on B cells. Expression of TNF-alpha has been detected in macrophages/monocytes, B-cells, T-cells, basophils, eosinophils, glial cells and mast cells. The effects of TNF-a are mediated through the TNF receptors, TNFRI (TNF R1) and TNFRII (TNF R2). TNFRI is a 55 kDa glycoprotein and contains 5 N-linked glycosylation sites.
Source : human 293 cells.
Molecular Mass : TNF RI-Fc Chimera migrates as a band between 50 and 60 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified TNF RI-Fc Chimera that has a predicted molecular mass of 48.0kDa.
PI : TNF RI-Fc Chimera separates into a number of isoforms with a pI between 6.5 and 8.6 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified TNF RI-Fc Chimera that has a predicted pI of 7.69.
% Carbohydrate : TNF RI-Fc Chimera consists of 5-20% carbohydrate by weight.
Glycosylation : TNF RI-Fc Chimera has N-linked and possibly O-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE and visualized by silver stain.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.
Activity : The ND50of TNF RI-Fc Chimera is typically 15-30 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D.
Gene Name : TNFRSF1A tumor necrosis factor receptor superfamily, member 1A [ Homo sapiens ]
Synonyms : tumor necrosis factor receptor superfamily, member 1A; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; MGC19588; TNFRSF1A; tumor necrosis factor receptor 1; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; TNF-R1; TNF-RI
Gene ID : 7132
mRNA Refseq : NM_001065
Protein Refseq : NP_001056
MIM : 191190
UniProt ID : P19438
Chromosome Location : 12p13.2
Pathway : Adipocytokine signaling pathway; Alzheimer"s disease; Amyotrophic lateral sclerosis (ALS); Apoptosis; Cytokine-cytokine receptor interaction; MAPK signaling pathway; Apoptosis
Function : protein complex binding; receptor activity; tumor necrosis factor receptor activity

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