Active Recombinant Human TNFRSF1A, Fc Chimera
Cat.No. : | TNFRSF1A-152H |
Product Overview : | Recombinant Humantumor necrosis factor receptor 1 was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modifiedhuman 293 cells. |
- Specification
- Gene Information
- Related Products
Cat. No. : | TNFRSF1A-152H |
Description : | Tumor necrosis factor alpha (TNF-a ; TNF alpha) and the structurally related lymphotoxin alpha are pro-inflammatory cytokines produced by a variety of immune and non-immune cells in response to multiple stimuli. These stimuli can include bacteria and bacterial products such as LPS, viral infections, T-cell receptor activation and cross-linking of surface immunoglobulin (Ig) on B cells. Expression of TNF-alpha has been detected in macrophages/monocytes, B-cells, T-cells, basophils, eosinophils, glial cells and mast cells. The effects of TNF-a are mediated through the TNF receptors, TNFRI (TNF R1) and TNFRII (TNF R2). TNFRI is a 55 kDa glycoprotein and contains 5 N-linked glycosylation sites. |
Source : | human 293 cells. |
Molecular Mass : | TNF RI-Fc Chimera migrates as a band between 50 and 60 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified TNF RI-Fc Chimera that has a predicted molecular mass of 48.0kDa. |
PI : | TNF RI-Fc Chimera separates into a number of isoforms with a pI between 6.5 and 8.6 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified TNF RI-Fc Chimera that has a predicted pI of 7.69. |
% Carbohydrate : | TNF RI-Fc Chimera consists of 5-20% carbohydrate by weight. |
Glycosylation : | TNF RI-Fc Chimera has N-linked and possibly O-linked oligosaccharides. |
Purity : | >95%, as determined by SDS-PAGE and visualized by silver stain. |
Formulation : | When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
Reconstitution : | It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
Storage : | Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. |
Activity : | The ND50of TNF RI-Fc Chimera is typically 15-30 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D. |
Gene Name : | TNFRSF1A tumor necrosis factor receptor superfamily, member 1A [ Homo sapiens ] |
Synonyms : | tumor necrosis factor receptor superfamily, member 1A; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; MGC19588; TNFRSF1A; tumor necrosis factor receptor 1; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; TNF-R1; TNF-RI |
Gene ID : | 7132 |
mRNA Refseq : | NM_001065 |
Protein Refseq : | NP_001056 |
MIM : | 191190 |
UniProt ID : | P19438 |
Chromosome Location : | 12p13.2 |
Pathway : | Adipocytokine signaling pathway; Alzheimer"s disease; Amyotrophic lateral sclerosis (ALS); Apoptosis; Cytokine-cytokine receptor interaction; MAPK signaling pathway; Apoptosis |
Function : | protein complex binding; receptor activity; tumor necrosis factor receptor activity |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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