Active Recombinant Human TNFRSF1A, Fc Chimera

• Cat.No.: TNFRSF1A-152H
• Product Overview: Recombinant Humantumor necrosis factor receptor 1 was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Description: Tumor necrosis factor alpha (TNF-a ; TNF alpha) and the structurally related lymphotoxin alpha are pro-inflammatory cytokines produced by a variety of immune and non-immune cells in response to multiple stimuli. These stimuli can include bacteria and bacterial products such as LPS, viral infections, T-cell receptor activation and cross-linking of surface immunoglobulin (Ig) on B cells. Expression of TNF-alpha has been detected in macrophages/monocytes, B-cells, T-cells, basophils, eosinophils, glial cells and mast cells. The effects of TNF-a are mediated through the TNF receptors, TNFRI (TNF R1) and TNFRII (TNF R2). TNFRI is a 55 kDa glycoprotein and contains 5 N-linked glycosylation sites.
• Molecular Mass: TNF RI-Fc Chimera migrates as a band between 50 and 60 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified TNF RI-Fc Chimera that has a predicted molecular mass of 48.0kDa.
• PI: TNF RI-Fc Chimera separates into a number of isoforms with a pI between 6.5 and 8.6 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified TNF RI-Fc Chimera that has a predicted pI of 7.69.
• % Carbohydrate: TNF RI-Fc Chimera consists of 5-20% carbohydrate by weight.
• Glycosylation: TNF RI-Fc Chimera has N-linked and possibly O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.
• Activity: The ND₅₀of TNF RI-Fc Chimera is typically 15-30 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D.

Gene Information

• Gene Name: TNFRSF1A tumor necrosis factor receptor superfamily, member 1A [ Homo sapiens ]
• Synonyms: tumor necrosis factor receptor superfamily, member 1A; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; MGC19588; TNFRSF1A; tumor necrosis factor receptor 1; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; TNF-R1; TNF-RI
• Gene ID: 7132
• mRNA Refseq: NM_001065
• Protein Refseq: NP_001056
• MIM: 191190
• UniProt ID: P19438
• Chromosome Location: 12p13.2
• Pathway: Adipocytokine signaling pathway; Alzheimer"s disease; Amyotrophic lateral sclerosis (ALS); Apoptosis; Cytokine-cytokine receptor interaction; MAPK signaling pathway; Apoptosis
• Function: protein complex binding; receptor activity; tumor necrosis factor receptor activity