HA Magnetic CoIP Agarose

• Cat.No.: HA-01A
• Product Overview: Immunoprecipitation of HA-fusion proteins with anti-HA Nanobody conjugated to magnetic agarose beads.
  • Fast, reliable & efficient one-stepImmunoprecipitation
  • Ready-to-use
  • No heavy & light antibody chains
  • Stable under harsh washing conditions
  • Suitable for downstream mass spec analysis
  • Works in samples from: mammals, plants, yeast, etc.

Specification

• Specificity/Target: Binds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low.
• Binding capacity: 20 ug recombinant HA-tagged protein (~30 kDa) per 25 uL bead slurry
• Conjugate: Magnetic agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: 2xSDS-sample buffer (Lammli)
• Wash buffer compatibility: 2M NaCl, 5 mM DTT, 0 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 2-3 M Urea
• Type: Nanobody
• Affinity (KD): 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions.
• Storage Buffer: 20% ethanol
• Applications: IP, Co-IP