Human DLAT Knockdown Cell Lysate

• Cat.No.: DLAT-137HKCL
• Product Overview: WB-validated DLAT Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 69 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg DLAT KD HeLa cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: DLAT dihydrolipoamide S-acetyltransferase [ Homo sapiens (human) ]
• Official Symbol: DLAT
• Synonyms: DLAT; dihydrolipoamide S-acetyltransferase; DLTA; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; E2 component of pyruvate dehydrogenase complex; PDC E2; PBC; M2 antigen complex 70 kDa subunit; pyruvate dehydrogenase complex component E2; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial; PDCE2; PDC-E2
• Gene ID: 1737
• mRNA Refseq: NM_001931
• Protein Refseq: NP_001922
• MIM: 608770
• UniProt ID: P10515