CCL25-70H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Human Chemokine (C-C motif) Ligand 25 was expressed inE. Coli.
Description :
Human TECK (thymus-expressed chemokine) is a novel CC chemokine that was identified int he thymus of mouse and human. The TECK shows chemotactic activity for activated macrophages, dendritic cells and thymocytes. This rec human TECK is a 14.2 kDa protein containing 127 amino acid residues.
Source :
E. Coli.
Amino Acid Sequence :
QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAAIFYLPKRHRK VC GN PK SREV QRAMKLLDAR NKVFAKLHHNMQTFQAGPHA VKKLSSGNSK LS SS K FS NPI SSSRKNVSLLISANSGL.
Supplied :
Lyophilized powder with no additives.
Purity :
>98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of TECK.
Reactivity :
The reactivity of this rhuman TECK was determined by a chemotaxis assay using human monocytes. Significant chemotaxis was achieved using concentration of 0.1-10ng/ml.
Note :
If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to further dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Reconstitution :
The lyophilized TECK should be reconstituted in water to a concentration of 0.1- 1.0mg/ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at -20 °C for future use.
Storage :
The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted TECK should be stored in working aliquots at -20°C. AVOID FREQUENT FREEZE THAW CYCLES.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.