Recombinant Human Chemokine (C-C motif) Ligand 25
Cat.No. : | CCL25-70H |
Product Overview : | Recombinant Human Chemokine (C-C motif) Ligand 25 was expressed inE. Coli. |
Availability | February 16, 2025 |
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Cat. No. : | CCL25-70H |
Description : | Human TECK (thymus-expressed chemokine) is a novel CC chemokine that was identified int he thymus of mouse and human. The TECK shows chemotactic activity for activated macrophages, dendritic cells and thymocytes. This rec human TECK is a 14.2 kDa protein containing 127 amino acid residues. |
Source : | E. Coli. |
Amino Acid Sequence : | QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAAIFYLPKRHRK VC GN PK SREV QRAMKLLDAR NKVFAKLHHNMQTFQAGPHA VKKLSSGNSK LS SS K FS NPI SSSRKNVSLLISANSGL. |
Supplied : | Lyophilized powder with no additives. |
Purity : | >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of TECK. |
Reactivity : | The reactivity of this rhuman TECK was determined by a chemotaxis assay using human monocytes. Significant chemotaxis was achieved using concentration of 0.1-10ng/ml. |
Note : | If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to further dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml. |
Reconstitution : | The lyophilized TECK should be reconstituted in water to a concentration of 0.1- 1.0mg/ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at -20 °C for future use. |
Storage : | The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted TECK should be stored in working aliquots at -20°C. AVOID FREQUENT FREEZE THAW CYCLES. |
Tag : | Non |
Publication : |
Early gene activation initiates neuroinflammation prior to VSV neuroinvasion: Impact on antiviral responses and sleep (2017)
|
Gene Name : | chemokine (C-C motif) ligand 25 [Homo sapiens] |
Synonyms : | chemokine (C-C motif) ligand 25; TECK; Ckb15; SCYA25; MGC150327; CCL25; small inducible cytokine A25; TECKvar; Ck beta-15; chemokine TECK; thymus expressed chemokine; small inducible cytokine subfamily A (Cys-Cys), member 25; TECKvar; Thymus-expressed chemokine; Small-inducible cytokine A25; Chemokine TECK |
Gene ID : | 6370 |
mRNA Refseq : | NM_005624 |
Protein Refseq : | NP_005615 |
MIM : | 602565 |
UniProt ID : | O15444 |
Chromosome Location : | 19p13.2 |
Pathway : | Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Signaling by GPCR |
Function : | chemokine activity; hormone activity; protein binding |
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