| Species : |
Yeast |
| Description : |
Alcohol oxidase (AOX) is a homooctamer with a molecular weight of 600 kDa. It contains eight flavin adenine dinucleotide (FAD) cofactors. It belongs to the glucose-methanol-choline (GMC) oxidoreductase family. |
| CAS : |
9073-63-6 |
| Molecular Mass : |
675 kDa (octomer, gel filtration) |
| Bio-activity : |
10-40 units/mg protein |
| Unit Definition : |
One unit will oxidize 1.0 mole of methanol to formaldehyde per minute at pH 7.5 at 25 centigrade. |
| Stability : |
It remains active for at least 2 years when stored at -20 centigrade. |
| Storage : |
At -20 centigrade |
| Storage Buffer : |
Buffered aqueous solution |
| Reconstitution : |
Alcohol oxidase is soluble in cold 100 mM potassium phosphate, pH 7.5, at 25 centigrade. Solutions should be prepared just before use. |
| Shipping : |
Dry Ice |
| Reference : |
1. Kato, N. et al., Agric. Biol. Chem., 38, 675 (1974).
2. Sahm, H. et al., Alcohol oxidase from Candida boidinii. Methods Enzymol., 89, 424-28 (1982).
3. Evers, M.E. et al., Assembly of alcohol oxidase in peroxisomes of the yeast Hansenula polymorpha requires the cofactor flavin adenine dinucleotide. Mol. Biol. Cell, 5, 829-37 (1994). |
