Active Native Pichia pastoris MOX Protein

• Cat.No.: MOX-01P
• Product Overview: This product is purified from Pichia pastoris and is supplied in a phosphate-buffered 30% sucrose solution. Cofactor: FAD, one molecule/subunit pH Range: 6.5-8.3 pH Optimum: 7.5 Temperature range: 18-45 centigrade Temperature optimum: 37 centigrade Inhibitor: H2O2

Specification

• Species: Yeast
• Description: Alcohol oxidase (AOX) is a homooctamer with a molecular weight of 600 kDa. It contains eight flavin adenine dinucleotide (FAD) cofactors. It belongs to the glucose-methanol-choline (GMC) oxidoreductase family.
• CAS: 9073-63-6
• Molecular Mass: 675 kDa (octomer, gel filtration)
• Bio-activity: 10-40 units/mg protein
• Unit Definition: One unit will oxidize 1.0 mole of methanol to formaldehyde per minute at pH 7.5 at 25 centigrade.
• Stability: It remains active for at least 2 years when stored at -20 centigrade.
• Storage: At -20 centigrade
• Storage Buffer: Buffered aqueous solution
• Reconstitution: Alcohol oxidase is soluble in cold 100 mM potassium phosphate, pH 7.5, at 25 centigrade. Solutions should be prepared just before use.
• Shipping: Dry Ice
• Reference: 1. Kato, N. et al., Agric. Biol. Chem., 38, 675 (1974). 2. Sahm, H. et al., Alcohol oxidase from Candida boidinii. Methods Enzymol., 89, 424-28 (1982). 3. Evers, M.E. et al., Assembly of alcohol oxidase in peroxisomes of the yeast Hansenula polymorpha requires the cofactor flavin adenine dinucleotide. Mol. Biol. Cell, 5, 829-37 (1994).

Gene Information

• Official Symbol: AOX
• Synonyms: EC 1.1.3.13; oxygen oxidoreductase; Alcohol Oxidase; AOX