Active Recombinant Mouse Fgf2 Protein

• Cat.No.: Fgf2-7206M
• Product Overview: Recombinant Mouse Fibroblast Growth Factor-2 without tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Description: Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
• Form: Lyophilized freeze dried protein without additives.
• Bio-activity: The activity was determined by the stimulation of HUVE cells. The ED50 for this effect is = 0.1 ng/mL corresponding to a specific activity of = 1 × 10^7 units/mg.
• Molecular Mass: 16.34 kDa
• AA Sequence: ALPEDGGAAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKGPGQKAILFLPMSAKSResultbyN-terminalsequencing:ALPEDGGAAF
• Endotoxin: < 0.1 ng/μg of Mouse FGF-2
• Purity: > 95 %
• Storage: The lyophilized Mouse FGF-2, though stable at room temperature, is best stored in working aliquots at –20 to –70 centigrade.
  Avoid repeated freeze-thaw cycles
• Storage Buffer: PBS without stabilizers
• Reconstitution: Restore with ddH2O or 50 mM Acetic Acid at 50 μg/mL. This solution can be diluted into other buffered solutions or stored frozen for future use.

Gene Information

• Gene Name: Fgf2 fibroblast growth factor 2 [ Mus musculus (house mouse) ]
• Official Symbol: Fgf2
• Synonyms: Fgf2; fibroblast growth factor 2; Fgfb; bFGF; Fgf-2; Fgf2a; fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; fibroblast growth factor 2a; heparin-binding growth factor 2
• Gene ID: 14173
• mRNA Refseq: NM_008006
• Protein Refseq: NP_032032
• UniProt ID: P15655