| Species : |
Mouse |
| Source : |
Insect Cells |
| Tag : |
His |
| Description : |
Glo1, also known as lactoylglutathione lyase, is a member of the glyoxalase l family. It plays a critical role in the detoxification of 2-oxoaldehydes, such as methylglyoxal. It involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. It was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. |
| Form : |
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
| Bio-activity : |
Specific activity is >210 units/mg, and is defined as the amount of enzyme that will form 1.0 µmol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25 centigrade. |
| Molecular Mass : |
21.8kDa (192aa)28-40kDa (SDS-PAGE under reducing conditions) |
| AA Sequence : |
MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTAWTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKIATIILEHHHH HH |
| Endotoxin : |
< 1.0 EU per 1 microgram of protein (determined by LAL method) |
| Purity : |
>95% by SDS - PAGE |
| Storage : |
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
0.5mg/ml (determined by Absorbance at 280nm) |
