| Species : |
Human |
| Source : |
HT-1080 |
| Tag : |
Non |
| Description : |
The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1,384-amino acid protein, also designated p190 or CASPR for 'contactin-associated protein,' includes an extracellular domain with several putative protein-protein interaction domains, a putative transmembrane domain, and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins, and this protein may be the signaling subunit of contactin, enabling recruitment and activation of intracellular signaling pathways in neurons. |
| Form : |
Cell-Tissue Lysis buffer |
| Molecular Mass : |
156 kDa |
| Notes : |
Instruction of use: This knockdown cell lysate should be paired with wild-type HT-1080 cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins. |
| Storage : |
Store at -20 centigrade for two years. |
| Concentration : |
Lot-specific |
| Shipping : |
Blue Ice |
| Components : |
1 vial of 100 μg WT HT-1080 cell lysate
1 vial of 100 μg CNTNAP1 KD HT-1080 cell lysate |
| Protein Families : |
Druggable Genome, Transmembrane |
| Protein Pathways : |
Cell adhesion molecules (CAMs) |
| Lysate QC : |
RT-qPCR; Western Blotting (WB) |
