| Species : |
Human |
| Source : |
HT-1080 |
| Tag : |
Non |
| Description : |
This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. |
| Form : |
Cell-Tissue Lysis buffer |
| Molecular Mass : |
33 kDa |
| Notes : |
Instruction of use: This knockdown cell lysate should be paired with wild-type HT-1080 cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins. |
| Storage : |
Store at -20 centigrade for two years. |
| Concentration : |
Lot-specific |
| Shipping : |
Blue Ice |
| Components : |
1 vial of 100 μg WT HT-1080 cell lysate
1 vial of 100 μg PABPN1 KD HT-1080 cell lysate |
| Protein Families : |
Druggable Genome |
| Lysate QC : |
RT-qPCR; Western Blotting (WB) |
