Recombinant Human ALPI Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: ALPI-024H
• Product Overview: ALPI MS Standard C13 and N15-labeled recombinant protein (NP_001622) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 56.81 kDa
• AA Sequence: MQGPWVLLLLGLRLQLSLGVIPAEE ENPAFWNRQAAEALDAAKKLQPIQK VAKNLILFLGDGLGVPTVTATRILK GQKNGKLGPETPLAMDRFPYLALSK TYNVDRQVPDSAATATAYLCGVKAN FQTIGLSAAARFNQCNTTRGNEVIS VMNRAKQAGKSVGVVTTTRVQHASP AGTYAHTVNRNWYSDADMPASARQE GCQDIATQLISNMDIDVILGGGRKY MFPMGTPDPEYPADASQNGIRLDGK NLVQEWLAKHQGAWYVWNRTELMQA SLDQSVTHLMGLFEPGDTKYEIHRD PTLDPSLMEMTEAALRLLSRNPRGF YLFVEGGRIDHGHHEGVAYQALTEA VMFDDAIERAGQLTSEEDTLTLVTA DHSHVFSFGGYTLRGSSIFGLAPSK AQDSKAYTSILYGNGPGYVFNSGVR PDVNESESGSPDYQQQAAVPLSSET HGGEDVAVFARGPQAHLVHGVQEQS FVAHVMAFAACLEPYTACDLAPPAC TTDAAHPVAASLPLLAGTLLLLGAS AAPTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ALPI alkaline phosphatase, intestinal [ Homo sapiens (human) ]
• Official Symbol: ALPI
• Synonyms: ALPI alkaline phosphatase, intestinal; IAP; intestinal-type alkaline phosphatase; Kasahara isozyme; alkaline phosphomonoesterase; glycerophosphatase; intestinal alkaline phosphatase; EC 3.1.3.1
• Gene ID: 248
• mRNA Refseq: NM_001631
• Protein Refseq: NP_001622
• MIM: 171740
• UniProt ID: P09923