Recombinant Human CLCN3 Protein, His-tagged

• Cat.No.: CLCN3-01H
• Product Overview: Recombinant Human CLCN3 Protein(NP_001230301.1), fused to His-tag, was expressed in E. coli.

Specification

• Description: This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from 20 mM Tris-HCl, 0.15 M NaCl, 0.05% Brij78, 6% Trehalose, pH 8.0.
  The volume before lyophilization is 100μl/vial.
• Molecular Mass: 93.2 kDa, reducing condition.
• AA Sequence: MESEQLFHRGYYRNSYNSITSASSD EELLDGAGVIMDFQTSEDDNLLDGD TAVGTHYTMTNGGSINSSTHLLDLL DEPIPGVGTYDDFHTIDWVREKCKD RERHRRINSKKKESAWEMTKSLYDA WSGWLVVTLTGLASGALAGLIDIAA DWMTDLKEGICLSALWYNHEQCCWG SNETTFEERDKCPQWKTWAELIIGQ AEGPGSYIMNYIMYIFWALSFAFLA VSLVKVFAPYACGSGIPEIKTILSG FIIRGYLGKWTLMIKTITLVLAVAS GLSLGKEGPLVHVACCCGNIFSYLF PKYSTNEAKKREVLSAASAAGVSVA FGAPIGGVLFSLEEVSYYFPLKTLW RSFFAALVAAFVLRSINPFGNSRLV LFYVEYHTPWYLFELFPFILLGVFG GLWGAFFIRANIAWCRRRKSTKFGK YPVLEVIIVAAITAVIAFPNPYTRL NTSELIKELFTDCGPLESSSLCDYR NDMNASKIVDDIPDRPAGIGVYSAI WQLCLALIFKIIMTVFTFGIKVPSG LFIPSMAIGAIAGRIVGIAVEQLAY YHHDWFIFKEWCEVGADCITPGLYA MVGAAACLGGVTRMTVSLVVIVFEL TGGLEYIVPLMAAVMTSKWVGDAFG REGIYEAHIRLNGYPFLDAKEEFTH TTLAADVMRPRRNDPPLAVLTQDNM TVDDIENMINETSYNGFPVIMSKES QRLVGFLRRDLTIAIESARKKQEGI VGSSRVCFAQHTPSLPAESPRPLKL RSILDMSPFTVTDHTPMEIVVDIFR KLGLRQCLVTHNGRLLGIITKKDIL RHMAQTANQDPASIMFNHHHHHH
• Purity: ≥85%, by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: CLCN3 chloride voltage-gated channel 3 [ Homo sapiens (human) ]
• Official Symbol: CLCN3
• Synonyms: CLC3; ClC-3
• Gene ID: 1182
• mRNA Refseq: NM_001243372.2
• Protein Refseq: NP_001230301.1
• MIM: 600580
• UniProt ID: P51790