| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein. |
| Bio-activity : |
The activity of 11β-HSD enzyme is defined as total turnover number (nmol product/nmol enzyme/min) during the in vitro conversion of cortisone to cortisol (hydrocortisone) in the presence of NADPH regenerating system (5 mM glucose-6 phosphate, 2U of glucose-6-phosphate dehydrogenase, and 0.5mM of magnesium chloride). The substrate and product were separated in a HPLC using a reverse phase C18-column detected at 245 nm. |
| Molecular Mass : |
31.4 kDa |
| Purity : |
≥75% estimated by SDS-PAGE |
| Stability : |
≥ 2 year |
| Storage : |
At -80 centigrade. |
| Storage Buffer : |
50 mM sodium phosphate buffer, pH 7.6, containing 100 mM sodium chloride and 20% glycerol |
