Active Recombinant Full Length Human BMI1 Protein, N-His-tagged

• Cat.No.: BMI1-427HFL
• Product Overview: Recombinant Full Length Human BMI1 Protein, fused to His-tag at N-terminus, was expressed in E. coli.

Specification

• Description: This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 100 mM Arg.
• Bio-activity: In vitro kinase assay substrate
  Binding assay (competitor)
• Molecular Mass: 36.9 kDa
• AA Sequence: A DNA sequence encoding human full-length BMI1.
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors

Gene Information

• Gene Name: BMI1 BMI1 proto-oncogene, polycomb ring finger [ Homo sapiens (human) ]
• Official Symbol: BMI1
• Synonyms: PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1
• Gene ID: 648
• mRNA Refseq: NM_005180.9
• Protein Refseq: NP_005171.4
• MIM: 164831
• UniProt ID: P35226