| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Non |
| Description : |
Interleukin-3 (IL-3) is a pleiotropic cytokine that regulates the proliferation, maturation, and survival of progenitor cells of the myeloid, erythroid, and megakaryocyte lineage. IL-3 is predominantly produced by activated T cells although expression has also been detected in monocytes and macrophages, NK-cells, mast cells, endothelial cells, and keratinocytes. |
| Amino Acid Sequence : |
APMTQTTSLKTS WVNCSNMID EIITHLKQPPL PLLDFNNLN GEDQDILME NNL RRP NLEA FNRAVKSLQN ASAIESILKNLL PCLPLATAAPTRH PIHIKDGDWNE FRRKLTFYLKTLENAQAQQTTLSLAIF. |
| Molecular Mass : |
IL-3 migrates as a broad band between 15 and 25 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-3 that has a predicted molecular mass of 15.1 kDa. |
| PI : |
IL-3 separates into a number of isoforms with a pI between 4.2 and 6.8 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-3 that has a predicted pI of 7.05. |
| % Carbohydrate : |
Purified IL-3 consists of 0-40% carbohydrate by weight. |
| Glycosylation : |
IL-3 has N-linked and possibly O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Product should be stored at 2 to 8°C. Short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| Activity : |
The ED50of IL-3 is typically 0.1- 0.4 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line. |
