Recombinant Human CD40 protein, Hi & Fc & Avi-tagged, Biotinylated


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Recombinant Human CD40 protein, Hi & Fc & Avi-tagged, Biotinylated

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Cat.No. : CD40-369H
Product Overview : Biotinylated Human CD40 / TNFRSF5, His Tag & Fc Tag is expressed from human HEK293 cells. It contains AA Glu 21 - Arg 193 (Accession # AAH12419.1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Description : CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines.
Source : HEK293
Species : Human
Tag : Hi & Fc & Avi
Predicted N Terminal : Glu 21
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity : Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 µl/well),can bind Biotinylated Human CD40, His Tag & Fc tag with a linear of 0.2-1 μg/mL.
Molecular Mass : This protein carries a polyhistidine tag at the C-terminus. The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 48 kDa. The protein migrates as 55-65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Protein length : Glu 21 - Arg 193
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Storage buffer : 50 mM Tris, 100 mM Glycine, pH7.5
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping : Powder,RT

Biotinylated Human CD40, His Tag & Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : CD40 CD40 molecule [ Homo sapiens (human) ]
Official Symbol : CD40
Synonyms : p50; Bp50; CDW40; TNFRSF5; B cell surface antigen CD40; B cell-associated molecule; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor
Gene ID : 958
mRNA Refseq : NM_001250.5
Protein Refseq : NP_001241.1
UniProt ID : P25942
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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