| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Avi&Fc&His |
| Protein Length : |
Glu 21 - Arg 193 |
| Description : |
CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. |
| Predicted N Terminal : |
Glu 21 |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose. |
| Bio-activity : |
Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 µl/well),can bind Biotinylated Human CD40, His Tag & Fc tag with a linear of 0.2-1 μg/mL. |
| Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus. The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 48 kDa. The protein migrates as 55-65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Storage buffer : |
50 mM Tris, 100 mM Glycine, pH7.5 |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Shipping : |
Powder,RT |
| Conjugation : |
Biotin |
