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Recombinant Human CD40 protein, Hi & Fc & Avi-tagged, Biotinylated

Cat.No.: CD40-369H
Product Overview: Biotinylated Human CD40 / TNFRSF5, His Tag & Fc Tag is expressed from human HEK293 cells. It contains AA Glu 21 - Arg 193 (Accession # AAH12419.1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Description: CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines.
Source: HEK293
Species: Human
Tag : Hi & Fc & Avi
Predicted N Terminal: Glu 21
Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity: Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 µl/well),can bind Biotinylated Human CD40, His Tag & Fc tag with a linear of 0.2-1 μg/mL.
Molecular Mass: This protein carries a polyhistidine tag at the C-terminus. The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 48 kDa. The protein migrates as 55-65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Protein length: Glu 21 - Arg 193
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Storage buffer: 50 mM Tris, 100 mM Glycine, pH7.5
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping: Powder,RT
Gene Name: CD40 CD40 molecule [ Homo sapiens (human) ]
Official Symbol: CD40
Synonyms: p50; Bp50; CDW40; TNFRSF5; B cell surface antigen CD40; B cell-associated molecule; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor
Gene ID: 958
mRNA Refseq: NM_001250.5
Protein Refseq: NP_001241.1
UniProt ID: P25942

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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