| Species : |
Human |
| Source : |
HeLa |
| Tag : |
Non |
| Description : |
This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Form : |
Cell-Tissue Lysis buffer |
| Molecular Mass : |
97 kDa |
| Notes : |
Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins. |
| Storage : |
Store at -20 centigrade for two years. |
| Concentration : |
Lot-specific |
| Shipping : |
Blue Ice |
| Components : |
1 vial of 100 μg WT HeLa cell lysate
1 vial of 100 μg DNM1 KD HeLa cell lysate |
| Protein Families : |
Druggable Genome |
| Protein Pathways : |
Endocytosis, Fc gamma R-mediated phagocytosis |
| Lysate QC : |
RT-qPCR; Western Blotting (WB) |
