| Cat.No. : |
TIGIT-121C
Optional Service: Optional requirements on this protein
|
| Product Overview : |
Recombinant Cynomolgus/Rhesus macaque TIGIT protein (Met89-Ile208)(HPLC-verified), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293). In the region Met89-Ile208, the AA sequence of Cynomolgus and Rhesus macaque TIGIT are homologus. |
| Description : |
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. |
| Source : |
HEK293 |
| Species : |
Cynomolgus/Rhesus macaque |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
| Molecular Mass : |
The protein has a calculated MW of 15.2 kDa. The protein migrates as 18-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : |
120 |
| Endotoxin : |
Less than 1.0 EU per ug by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE of TIGIT-121C : |
Cynomolgus/Rhesus macaque TIGIT, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|
| SEC-HPLC of TIGIT-121C : |
The purity of Cynomolgus/Rhesus macaque TIGIT, His Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC.
|
| Bioactivity-ELISA of TIGIT-121C : |
Immobilized Cynomolgus/Rhesus macaque TIGIT, His Tag (HPLC-verified) at 5 μg/mL (100 μL/well) can bind Human CD155, Fc Tag with a linear range of 0.078-2.5 μg/mL (QC tested).
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.