Embryonic Stem Cells Proteins


 Embryonic Stem Cells Proteins Background

Embryonic stem cells are a type of cells isolated from early embryos (before the gut stage) or primitive gonads. They have the characteristics of in vitro proliferation, self-renewal and multi-directional differentiation in vitro. ES cells can be induced to differentiate into almost all cell types in the body, both in vitro and in vivo. Embryonic stem cell research has been a controversial area in the United States, and supporters believe that this study can help cure many intractable diseases, because embryonic stem cells can differentiate into multiple functional APSC pluripotent cells, which is considered to be a life-saving the charitable act is a manifestation of scientific progress. Opponents argue that embryonic stem cell research must destroy the embryo, and the embryo is the life form of the uterus when the person has not yet formed,which have anti-life ethics.

Embryonic stem cells Figure 1. Embryonic stem cells.

Introductions

Human ES cells can also differentiate into trophoblast cells, nerve cells, glial cells, hematopoietic cells, cardiomyocytes, and the like. ES cells can be used not only as a model for studying cell differentiation and developmental regulation mechanisms in vitro, but also as a vector for introducing site-directed mutagenesis of genomes generated by homologous recombination into individuals, and more importantly, ES cells will be transplanted to humans. Medicine brings a revolution. Further, embryonic stem cells (ES cells) are highly undifferentiated cells. It has developmental pluripotency and can differentiate into all tissues and organs of adult animals, including germ cells. Research and utilization of ES cells is one of the core issues in the current bioengineering field. The study of ES cells can be traced back to the 1950s. The discovery of teratoma stem cells (EC cells) began the biological research process of ES cells.

Human embryonic stem cells in cell culture. Figure 2. Human embryonic stem cells in cell culture.

Morphological characteristics

ES cells have a similar morphological structure to early embryonic cells. The nucleus is large, with one or several nucleoli. The nucleus is mostly euchromatin, with little cytoplasmic cytoplasm and simple structure. When cultured in vitro, the cells are arranged closely and grow in a colony. When stained with alkaline phosphatase, the ES cells were brownish red and the surrounding fibroblasts were pale yellow. There is a clear boundary between the cell clone and the surrounding, and the formed clonal cells are unclear with each other, and the cell surface has fat droplets with strong refraction. Cell clones are diverse in morphology, most of which are island-shaped or nest-like. Mouse ES cells are 7 microns to 18 microns in diameter, and pig, cow, and sheep ES cells are darker in color and 12 to 18 microns in diameter.

Differentiation characteristics

  1. All-round

    The pluripotency of ES cells means that ES cells can participate in the developmental potential of various tissues including the gonads under the condition of dedifferentiation inhibition, that is, ES cells have the ability to develop into a complete animal body, which can be genetic manipulation and cell differentiation of cells. The research provides a wealth of experimental materials. The hallmark of ES cell development pluripotency is the expression of the phase specific embryonicant (SSEA) on the surface of ES cells, and the expression of the Oct4 gene, which is a marker of developmental pluripotency, can be examined. ES cells have high AKP and telomerase activity and can be used for the identification of ES cell differentiation. Totipotency is the main difference between embryonic stem cells and pluripotent stem cells in the body. Under normal culture conditions, embryonic stem cells remain versatile after multiple cell divisions.

  2. Pluripotent

    The pluripotency of ES cells means that ES cells have the ability to develop into a variety of tissues and participate in the formation of some tissues. Embryoid bodies can be formed by culturing ES cells on a medium containing no differentiation inhibitor. ES cells can be cultured in a specific medium and differentiated into specific tissues. For example, ES cells can differentiate into whole-wall endoderm on ES medium containing leukemia inhibitory factor (LIF) and vitamin A (RA). Cells are co-cultured with embryonic cells or ES cells are injected into the blastocyst cavity, and ES cells participate in the development of various tissues.

Pluripotent of embryonic stem cells. Figure 3. Pluripotent of embryonic stem cells.

Reference:

  1. Thomson, J. A.; et al. Embryonic Stem Cell Lines Derived from Human Blastocysts. Anatomy and Embryology. Science. 1998, 282 (5391): 1145–1147.