Active Recombinant Mouse IL23 protein(Met1-Ala196 & Met1-Ser335), His-tagged

• Cat.No.: IL23-3392M
• Product Overview: Recombinant Mouse IL23A (Q9EQ14) (Met1-Ala196) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding Mouse IL12B (P43432) (Met1-Ser335) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Mouse IL23 heterodimer was purified.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ala196 & Met1-Ser335
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized Human IL23 Receptor hFc at 2 μg/ml (100 μl/well) can bind Mouse IL-23 His, the EC50 of Mouse IL-23 His is 50-250 ng/mL. 2. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-4 ng/mL.
• Molecular Mass: The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.