| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag with a linear range of 0.02-0.313 μg/mL.Immobilized Human IL-23 R, Fc Tag at 10 μg/mL (100 μL/well) can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with a linear range of 0.039-0.313 μg/mL. |
| Molecular Mass : |
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23A and IL-12B, has a calculated MW of 20.5 kDa (IL-23A) and 35.8 kDa (IL-12B). Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The reducing (R) protein migrates as 19 kDa (IL-23A) and 40-44 kDa (IL-12B) respectively due to glycosylation. |
| Proteinlength : |
Val22-Ala196(IL23A)&Met23-Ser335(IL12B) |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
