| Description : |
IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. |
| Source : |
E.Coli |
| Species : |
Rat |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1mg/ml) solution in water containing 0.4 x PBS pH=7.2. |
| Bio-activity : |
The ED50 as determined by the dose-dependant stimulation of IFN-gamma by murine lymph node cells (400,000cells/well) co-stimulated with sub optimal dose of ConA (0.75ug/ml) is 12ng/ml corresponding to a specific activity of 84,000U/mg. The medium was RPMI containing 10% FCS and 50µM 2-ME. |
| AA Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be His-Phe-Gly-Arg-Leu. |
| Purity : |
Greater than 95.0% as determined by RP-HPLC and SDS-PAGE. |
| Stability : |
Lyophilized Interleukin-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
