|Product Overview :||Recombinant Human IL15 Protein is expressed from E.coli cells. It contains AA Asn 49 - Ser 162 (Accession # AAH18149). Predicted N-terminus: Asn 49. This protein carries no "tag". The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity :||Human IL-2 R beta, Fc Tag captured on CM5 chip via anti-human IgG Fc antibody, can bind Recombinant Human IL15 Protein with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (QC tested).
Recombinant Human IL15 Protein stimulates proliferation of CTLL-2 cells. The ED50 for this effect is 0.097-0.19 ng/ml (Routinely tested).
|Molecular Mass :||12.8 kDa|
|Protein length :||Asn 49 - Ser 162|
|Endotoxin :||Less than 0.1 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Notes :||Please avoid repeated freeze-thaw cycles.|
|Stability :||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name :||IL15 interleukin 15 [ Homo sapiens ]|
|Official Symbol :||IL15|
|Synonyms :||IL15; interleukin 15; interleukin-15; IL 15; MGC9721; IL-15;|
|Gene ID :||3600|
|mRNA Refseq :||NM_000585|
|Protein Refseq :||NP_000576|
|UniProt ID :||P40933|