Active Recombinant Mouse Il27 protein, His-tagged

• Cat.No.: Il27-1309M
• Product Overview: Recombinant Mouse Il27(His193~Val296) fused with His tag at C-terminal was expressed in HEK293.

Specification

• Description: Recombinant Mouse Il27 complex composed of EBI3 (O35228) (Met1-Pro228) and IL27A (Q8K3I6) (Phe29-Ser234) linked by an polypeptide linker was fused with the polyhistidine tag at the C-terminus.
• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: Tyr 19
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc, The EC50 of human IL27RA-Fc is 0.26-0.62 μg/ml.
  2. Measured in antiviral assay using HepG2 cells infected with vesicular stomatitisvirus (VSV).
  The ED50 for this effect is typically 0.07-0.42 μg/mL.
• Molecular Mass: The recombinant mouse IL27 comprises 442 amino acids and has a predicted molecular mass of 49.5 kDa. The apparent molecular mass of the protein is approximately 60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Il27 interleukin 27 [ Mus musculus ]
• Official Symbol: Il27
• Synonyms: IL27; interleukin 27; interleukin-27 subunit alpha; IL27-A; IL-27-A; interleukin 30; IL-27 p28 subunit; IL-27 subunit alpha; p28; Il30; IL-27; IL-27p28
• Gene ID: 246779
• mRNA Refseq: NM_145636
• Protein Refseq: NP_663611
• UniProt ID: Q8K3I6
• Chromosome Location: 7; 7 F3
• Function: cytokine activity; interleukin-27 receptor binding; protein binding; receptor binding