Active Recombinant Human IL2 Protein, His-tagged(C-ter)

• Cat.No.: IL2-169H
• Product Overview: Recombinant Active Human IL2 Protein with His tag (C-ter) was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
• Source: E. coli
• Species: Human
• Tag: His(C-ter)
• Form: Powder
• Bio-activity: Determined by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant human IL-2 is approximately > 2.5 x 10^7 IU/mg. Determined by its ability to induce proliferation in NK cells. The ED50 for this effect is < 46 ng/mL.
• AA Sequence: MAPTSSSTKKTQLQLEHLLLDLQMI LNGINNYKNPKLTRMLTFKFYMPKK ATELKHLQCLEEELKPLEEVLNLAQ SKNFHLRPRDLISNINVIVLELKGS ETTFMCEYADETATIVEFLNRWITF CQSIISTLT
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 98% (by SDS-PAGE)
• Applications: SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: PBS (pH 8.0)
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568

Reviews

04/13/2022

Potent in T-cell activation.

07/27/2018

Suitable for cytokine assays.

01/07/2018

High stability.

Q&As

What is the impact of IL2 on T-cell memory formation?

IL2 promotes the formation of memory T-cells.

What is the role of IL2 in the tumor microenvironment?

IL2 has a pro-tumorigenic role in the tumor microenvironment.

How does IL2 interact with other cytokines in a mixed environment?

IL2 synergizes with IL-12 in a mixed cytokine environment.

Does IL2 modulate the expression of checkpoint inhibitors?

IL2 upregulates the expression of PD-1 checkpoint inhibitors.

What are the kinetics of IL2 internalization in T cells?

IL2 is rapidly internalized by activated T-cells.

Does IL2 influence the polarization of macrophages?

IL2 induces M1 macrophage polarization.

How does IL2 affect the blood-brain barrier permeability?

IL2 increases blood-brain barrier permeability.

How does IL2's affinity for its receptor change under hypoxic conditions?

IL2's affinity for its receptor decreases under hypoxic conditions.

What post-translational modifications affect IL2's bioactivity?

IL2's bioactivity is enhanced by glycosylation.

What is the role of IL2 in the differentiation of Th17 cells?

IL2 promotes the differentiation of Th17 cells.