Recombinant Human IL12B, His-tagged

• Cat.No.: IL12B-43H
• Product Overview: Recombinant Human Interleukin-12 p40 His produced inE.Coliis single, a non-glycosylated, polypeptide chain containing 306 amino acids fragment (23-328) with an amino-terminal His-tagged. The IL-12 p40 His is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: IL12B-43H
• Description: Active IL-12 is a p70 disulphide-linked dimer composed of p35 and p40 subunits. The protein is a pleiotropic cytokine produced primarily by antigen presenting cells and has multiple effects on T lymphocytes and natural killer cells in terms of stimulating cytotoxicity, proliferation, production of other cytokines and Th1 subset differentiation.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered clear solution (70µg/ml).
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Interleukin-12 p40 His is supplied in 20mM Tris HCL pH=8 and 50% glycerol.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.

Gene Information

• Gene Name: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
• Synonyms: NKSF2; CTL maturation factor (TCMF); Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40); TSF; Edodekin-alpha; IL-12 p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; CLMF; CLMF2; IL-12B; IL12B; NKSF; OTTHUMP00000160820; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12, subunit p40; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; interleukin 12B; interleukin-12 beta chain; natural killer cell stimulatory factor-2
• Gene ID: 3593
• mRNA Refseq: NM_002187
• Protein Refseq: NP_002178
• MIM: 161561
• UniProt ID: P29460
• Chromosome Location: 5q31.1-q33.1
• Pathway: Allograft rejection; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; RIG-I-like receptor signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus
• Function: contributes_to cytokine activity; cytokine receptor activity; contributes_to growth factor activity; interleukin-12 alpha subunit binding; interleukin-12 receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; signal transducer activity

Reviews

04/08/2020

Good for cytokine assays.

01/06/2020

Effective in Th2 cell differentiation.

01/12/2019

High yield in expression.

Q&As

Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation?

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies?

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners?

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function?

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context?

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.