Active Recombinant Human IL2

• Cat.No.: IL2-2741H
• Product Overview: Recombinant human IL-2 produced in Yeast is a single, non-glycosylated, polypeptide chain containing 133 amino acids, one pair of disulfide bond and with 125 Cys. aa. mutated to Ala aa, having a molecular mass of approximately 15.4kDa.

Specification

• Description: Human IL-2 (also known as TCGF) is an about 15.4KD factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active. Recombinant human IL-2 is biologically active and can promote proliferation of T lymphocytes in culture.
• Source: Yeast
• Species: Human
• Form: Lyophilized from a 0.2μm filtered solution in 10mM Phosphate buffer containing 0.3% human serum albumin.
• Bio-activity: Measured in a cell proliferation assay using an IL-2 dependent Mouse cytotoxic T cell line, CTLL-2. The specific activity shall be not less than 3×10^7 IU/mg.
• Endotoxin: ≤1EU/μg, determined by the LAL method
• Purity: ≥97%, as determined by SDS-PAGE and HPLC method.
• Usage: FOR RESEARCH ONLY
• Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial, to prepare a stock solution of not less than 1μg/ml.

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens ]
• Official Symbol: IL2
• Synonyms: IL-2; TCGF; lymphokine; interleukin-2; T cell growth factor; aldesleukin; involved in regulation of T-cell clonal expansion
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568
• Chromosome Location: 4q26-q27
• Pathway: AhR pathway, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem
• Function: carbohydrate binding; cytokine activity; glycosphingolipid binding

Reviews

04/13/2022

Potent in T-cell activation.

07/27/2018

Suitable for cytokine assays.

01/07/2018

High stability.

Q&As

What is the impact of IL2 on T-cell memory formation?

IL2 promotes the formation of memory T-cells.

What is the role of IL2 in the tumor microenvironment?

IL2 has a pro-tumorigenic role in the tumor microenvironment.

How does IL2 interact with other cytokines in a mixed environment?

IL2 synergizes with IL-12 in a mixed cytokine environment.

Does IL2 modulate the expression of checkpoint inhibitors?

IL2 upregulates the expression of PD-1 checkpoint inhibitors.

What are the kinetics of IL2 internalization in T cells?

IL2 is rapidly internalized by activated T-cells.

Does IL2 influence the polarization of macrophages?

IL2 induces M1 macrophage polarization.

How does IL2 affect the blood-brain barrier permeability?

IL2 increases blood-brain barrier permeability.

How does IL2's affinity for its receptor change under hypoxic conditions?

IL2's affinity for its receptor decreases under hypoxic conditions.

What post-translational modifications affect IL2's bioactivity?

IL2's bioactivity is enhanced by glycosylation.

What is the role of IL2 in the differentiation of Th17 cells?

IL2 promotes the differentiation of Th17 cells.