Active Recombinant Human Interleukin 2, His-tagged

• Cat.No.: IL2-8861H
• Product Overview: Recombinant Human Interleukin 2 was produced in the endosperm tissue of barley grain (Hordeum vulgare).

Specification

• Description: Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL-2 may be of use as an anti-tumor agent in cancer therapy.
• Source: Hordeum vulgare
• Species: Human
• Predicted N Terminal: His
• Form: Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2.
• Bio-activity: Bioactivity of recombinant human IL-2 is assayed by measuring its dose dependent effect on proliferation of CTLL-2 cells. The ED50 for this effect is typically< 0.25 ng/ml, corresponding to a specific activity>4 x10e6 U/mg.
• Molecular Mass: Recombinant human IL-2 contains 133 amino acids and a 16 a.a. histidine-based tag for a total length of 149 a.a. and has a predicted molecular mass of 17.5 kDa. The recombinant protein migrates with an apparent molecular mass of 22 kDa in SDS-PAGE.
• Endotoxin: Endotoxin level is less than 0.05EU/μg as measured by turbidimetric kinetic assay.
• Purity: >95% by SDS-PAGE gel analysis.
• Storage: The lyophilized protein is stable at room temperature for few weeks. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568
• Chromosome Location: 4q26-q27
• Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem
• Function: carbohydrate binding; cytokine activity; glycosphingolipid binding; growth factor activity; interleukin-2 receptor binding; interleukin-2 receptor binding; kappa-type opioid receptor binding; kinase activator activity

Reviews

04/13/2022

Potent in T-cell activation.

07/27/2018

Suitable for cytokine assays.

01/07/2018

High stability.

Q&As

What is the impact of IL2 on T-cell memory formation?

IL2 promotes the formation of memory T-cells.

What is the role of IL2 in the tumor microenvironment?

IL2 has a pro-tumorigenic role in the tumor microenvironment.

How does IL2 interact with other cytokines in a mixed environment?

IL2 synergizes with IL-12 in a mixed cytokine environment.

Does IL2 modulate the expression of checkpoint inhibitors?

IL2 upregulates the expression of PD-1 checkpoint inhibitors.

What are the kinetics of IL2 internalization in T cells?

IL2 is rapidly internalized by activated T-cells.

Does IL2 influence the polarization of macrophages?

IL2 induces M1 macrophage polarization.

How does IL2 affect the blood-brain barrier permeability?

IL2 increases blood-brain barrier permeability.

How does IL2's affinity for its receptor change under hypoxic conditions?

IL2's affinity for its receptor decreases under hypoxic conditions.

What post-translational modifications affect IL2's bioactivity?

IL2's bioactivity is enhanced by glycosylation.

What is the role of IL2 in the differentiation of Th17 cells?

IL2 promotes the differentiation of Th17 cells.