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Recombinant Rat Il13

Cat.No.: Il13-580R
Product Overview: Recombinant Rat Il13 produced in E. coli is approximately 12.3 kDa, a single non-glycosylated polypeptide chain containing 113 amino acids.
Description: Interleukin-13 (IL-13) is expressed by the IL13 gene and secreted by many cell types, especially T helper type 2 (Th2) cells. The high solution from of IL-13 reported to be a monomer with two internal disulfide bonds that contribute to a bundled four α-helix configuration. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta, TNF-alpha, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Mature rat IL-13 shares 59 %, 75 %, and 60 % amino acid sequence identity with human, mouse, and rhesus IL-13, respectively.
Source: E. coli
Species: Rat
Form: Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Bio-activity: Fully biologically active when compared to standard. The ED 50 determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of >5.0 × 10^6 IU/mg.
Molecular Mass: 12.3 kDa
AA Sequence: TPGPVRRSTS PPVALRELIE ELSNITQDQK TSLCNSSIVW SVDLTAGGFC AALESLTNIS SCNAIHRTQR ILNGLCNQKA SDVASSPPDT KIEVAQFISK LLNYSKQLFR YGH
Endotoxin: Less than 1 EU/μg of rRtIL-13, 113a.a. as determined by LAL method.
Purity: >98% by SDS-PAGE and HPLC analyses.
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name: Il13 interleukin 13 [ Rattus norvegicus ]
Official Symbol: Il13
Synonyms: IL13; interleukin 13; interleukin-13; IL-13; T-cell activation protein P600;
Gene ID: 116553
mRNA Refseq: NM_053828
Protein Refseq: NP_446280
Pathway: Asthma, organism-specific biosystem; Asthma, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response (BioCarta), organism-specific biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem; Fc epsilon RI signaling pathway, conserved biosystem;
Function: cytokine activity; interleukin-13 receptor binding;

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